Protein Info for CSW01_13555 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional aspartate kinase/homoserine dehydrogenase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00696: AA_kinase
  • amino acids 5 to 277 (273 residues), 144.9 bits, E=5.2e-46
• TIGR00657: aspartate kinase
  • amino acids 8 to 445 (438 residues), 229.4 bits, E=4.5e-72
• PF03447: NAD_binding_3
  • amino acids 457 to 593 (137 residues), 57.3 bits, E=3.8e-19
• PF00742: Homoserine_dh
  • amino acids 601 to 796 (196 residues), 147.3 bits, E=6.5e-47

Best Hits

Swiss-Prot: 60% identical to AK2H_ECOLI: Bifunctional aspartokinase/homoserine dehydrogenase 2 (metL) from Escherichia coli (strain K12)

KEGG orthology group: K12525, bifunctional aspartokinase / homoserine dehydrogenase 2 [EC: 1.1.1.3 2.7.2.4] (inferred from 100% identity to vcm:VCM66_2604)

MetaCyc: 60% identical to fused aspartate kinase/homoserine dehydrogenase 2 (Escherichia coli K-12 substr. MG1655)
Aspartate kinase. [EC: 2.7.2.4]; Homoserine dehydrogenase. [EC: 2.7.2.4, 1.1.1.3]

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• norspermidine biosynthesis (6/6 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• ectoine biosynthesis (5/5 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• superpathway of polyamine biosynthesis III (7/8 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-homoserine biosynthesis (3/3 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• spermidine biosynthesis II (3/4 steps found)
• L-lysine biosynthesis II (6/9 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• L-methionine biosynthesis IV (2/4 steps found)
• 3-dehydroquinate biosynthesis II (archaea) (3/7 steps found)
• cremeomycin biosynthesis (2/7 steps found)
• grixazone biosynthesis (2/8 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3, 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Protein Sequence (803 amino acids)

>CSW01_13555 bifunctional aspartate kinase/homoserine dehydrogenase II (Vibrio cholerae E7946 ATCC 55056)
MTHPRQLHKFGGSSLADPECYQRVAKILKSYSKSDDLVVVSAAGKTTNRLISFVEALSKD
GRVAHETLHALRQYQSELITKLLSNEAAEPLLSQLQQEISVLGELTAPLSNAQYAWVLGH
GELWSARLLAALLNQQDLPAVAQDARTFLRAEAGTQPEVDRARSYPLLKAVLAQHTQRRV
VITGFMAQNEQGDTVLLGRNGSDYSATVIGALAEVSRVTIWSDVAGVYSADPRIVSDACL
LPLLRLDEANELARLAAPVLHSRTLQPVAQSTMELHLRCSHQPESGSTRIERVLASGRGA
KIITSLDDVLLIELSFAHHHDFQRVQEDVLQHLQRVQLQPLTYEAQPDQYRLRLAYTAEI
APGAFAALQDAAFEAEIKLKEGYDLIAAVGAGVTKNPNHCYGFYQQLNALPVEFISASES
SLSLVAVLRQTPIHSLVNAIHKQLFQAQKHVAIALCGKGNIGSSWLKLFAEQKEKLEQRH
GMNFELVAVVDSQTYWFNEQGINPNQVATHFQDEALPNQEQSWLKKLGALEGYDEAVVID
VTASEELAEQYLDIAEHGLHLISANKVAGSAAGNYYYQVKDAFHKIGRHWLYNATVGAGL
PINHTVRDLRESGDDIMALSGIFSGTLSWLFQQYDGSLPFSELVDLAWQQGLTEPDPRCD
LDGSDVMRKLVILARESGLEIEPQNVKVESLVPAELRSVSLDDFLDNSKLLNEQLAERLA
RAQKQGKVLRYVARLEKNGKASVGVEALEQDHPLANLLPCDNIFAIESKWYRDNPLVIRG
PGAGREVTAGAIQSDLNLLASLL