Protein Info for CSW01_13250 in Vibrio cholerae E7946 ATCC 55056

Annotation: aspartate aminotransferase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03246: succinylornithine transaminase family
  • amino acids 7 to 402 (396 residues), 694.1 bits, E=5.7e-213
• TIGR00707: transaminase, acetylornithine/succinylornithine family
  • amino acids 16 to 399 (384 residues), 494.3 bits, E=2.1e-152
• PF00202: Aminotran_3
  • amino acids 21 to 399 (379 residues), 437.4 bits, E=4.5e-135
• PF00155: Aminotran_1_2
  • amino acids 165 to 326 (162 residues), 22.1 bits, E=8.2e-09

Best Hits

Swiss-Prot: 100% identical to ARGD_VIBCH: Acetylornithine aminotransferase (argD) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00821, acetylornithine/N-succinyldiaminopimelate aminotransferase [EC: 2.6.1.11 2.6.1.17] (inferred from 100% identity to vcj:VCD_001745)

MetaCyc: 64% identical to succinylornithine transaminase subunit (Pseudomonas aeruginosa)
Succinylornithine transaminase. [EC: 2.6.1.81]; Ornithine aminotransferase. [EC: 2.6.1.81, 2.6.1.13]; Acetylornithine transaminase. [EC: 2.6.1.81, 2.6.1.13, 2.6.1.11]

Predicted SEED Role

"Acetylornithine aminotransferase (EC 2.6.1.11) / N-succinyl-L,L-diaminopimelate aminotransferase (EC 2.6.1.17) / Succinylornithine transaminase (EC 2.6.1.81)" in subsystem Arginine Biosynthesis extended or Lysine Biosynthesis DAP Pathway or Arginine and Ornithine Degradation (EC 2.6.1.11, EC 2.6.1.17, EC 2.6.1.81)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-arginine degradation XIII (reductive Stickland reaction) (5/5 steps found)
• L-ornithine biosynthesis I (5/5 steps found)
• L-ornithine biosynthesis II (3/3 steps found)
• L-proline biosynthesis III (from L-ornithine) (3/3 steps found)
• superpathway of arginine and polyamine biosynthesis (13/17 steps found)
• L-arginine degradation II (AST pathway) (4/5 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-arginine degradation VI (arginase 2 pathway) (3/4 steps found)
• L-arginine degradation I (arginase pathway) (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.11 or 2.6.1.13 or 2.6.1.17 or 2.6.1.81

Protein Sequence (403 amino acids)

>CSW01_13250 aspartate aminotransferase family protein (Vibrio cholerae E7946 ATCC 55056)
MTVEMSVDRSSFDEVMVPCYNPMEFIPVKGFGSRIWDQQGHEYIDFAGGIAVSCLGHCHP
VMVQALTTQANKLWHLSNVMTNEPALRLAKKLTQVSFAEKVFFANSGAEANEAALKLARR
YAADVYGPEKSEIIAFNQGFHGRTFFTVSVGGQATYSDGFGPKPGDIVHLPYNDLAALQA
QISDRTCAVMMEPLQGEGGIVSPSAEFVQAVRELCDKHNALLIFDEVQTGNGRTGDFYAY
QGIGVTPDILATAKSLGGGFPIGAMLTTAKIAEHMKVGVHGSTYGGNPLACAVAEAVVDF
VAQPEILAGVKQREQWMRAELEKINQKYQLFKEIRGKGLLLGAALNDEWQGRARDILVAA
GKQGLMVLVAGASVVRFTPSLIISQQEIEEGMARLDKAIATLM