Protein Info for CSW01_12970 in Vibrio cholerae E7946 ATCC 55056

Annotation: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 653 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01390: 2',3'-cyclic-nucleotide 2'-phosphodiesterase" amino acids 29 to 653 (625 residues), 1232.9 bits, see alignment E=0 PF00149: Metallophos" amino acids 30 to 266 (237 residues), 53.5 bits, see alignment E=4.3e-18 PF02872: 5_nucleotid_C" amino acids 429 to 553 (125 residues), 67.1 bits, see alignment E=1.9e-22

Best Hits

Swiss-Prot: 66% identical to CPDB_YEREN: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (cpdB) from Yersinia enterocolitica

KEGG orthology group: K01119, 2',3'-cyclic-nucleotide 2'-phosphodiesterase [EC: 3.1.4.16] (inferred from 100% identity to vco:VC0395_A2139)

MetaCyc: 66% identical to 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (Escherichia coli K-12 substr. MG1655)
2',3'-cyclic-nucleotide 2'-phosphodiesterase. [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3'-nucleotidase. [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]

Predicted SEED Role

"2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)" in subsystem Purine conversions (EC 3.1.4.16)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.16

Use Curated BLAST to search for 3.1.3.6 or 3.1.4.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (653 amino acids)

>CSW01_12970 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (Vibrio cholerae E7946 ATCC 55056)
MTMMIKPVSLAVLGGLLAMAGPVMADTIKLRIVETTDIHTNLMDYDYYKDKASDQIGLTR
AATLVKQARAEVENSVLVDNGDLIQGSPMGDYMAAKGIKAGEVHPVYKAMNPLGYDVGNI
GNHEFNYGLEFLKETINDAAFPYINANVFDAKTGQHLFTPYVIKEHSFKDTDGEAHTIKV
GYIGFVPPQIMVWDKKNLEGKVTAADIKQTAEKLIPEMKAKGADVIVAIPHSGISTDEYQ
AGAENSVYYLTQVKGIDAIAFGHSHAVFPGKDFANVPGADIDKGTINGVTAVMPGRWGSH
VGVMDLTLEKQQGRWAVTSGQSEARPIFDKANKKALVEADATMVQALQHDHQGTRDFVNQ
PIGKANDVMYSFLSLVQDDPTVQIVNLAQKDYVERFIQGDPNLDGLPVLSAAAPFKAGGR
KDDPNGFTEVEAGELTFRNAADLYLYPNTLVTMKVSGQQLKEWLECSAGQFNQIDVTSSA
PQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYDGDCKLINPNSQRIVQLTYQGKPIDTQ
QTFLIATNNYRAYSNKFPGTGAEFVAFDSPDENRTVVADYIARVSKDKGEVTPSADNNWS
FAPISSKTKLDIRFETSPSDKATQFIKQKGQYPMTRVASDDVGFAVYRIDLQK