Protein Info for CSW01_12845 in Vibrio cholerae E7946 ATCC 55056

Annotation: metalloprotease PmbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 transmembrane" amino acids 242 to 263 (22 residues), see Phobius details PF01523: PmbA_TldD_1st" amino acids 32 to 96 (65 residues), 46 bits, see alignment E=7.2e-16 PF19290: PmbA_TldD_2nd" amino acids 124 to 231 (108 residues), 68.1 bits, see alignment E=1.5e-22 PF19289: PmbA_TldD_3rd" amino acids 238 to 446 (209 residues), 245.9 bits, see alignment E=4e-77

Best Hits

Swiss-Prot: 64% identical to PMBA_ECO57: Metalloprotease PmbA (pmbA) from Escherichia coli O157:H7

KEGG orthology group: K03592, PmbA protein (inferred from 100% identity to vcm:VCM66_2456)

MetaCyc: 64% identical to metalloprotease subunit TldE (Escherichia coli)
3.4.24.-

Predicted SEED Role

"TldE protein, part of TldE/TldD proteolytic complex"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (447 amino acids)

>CSW01_12845 metalloprotease PmbA (Vibrio cholerae E7946 ATCC 55056)
MDVKERVALQRQELEAAVAKALELAAASSDAAEVAITKSTGLSVSTRMCEVENVEFNSDG
ALGITVYRGQRKGSASTSDLSEKAIRQTVLAALDIAQYTSADPYAGPAPKELMVQEIPDL
DLFHPDEPNPDVAAQIAIAAERAALGYSKKIKQSDGGSYDSHYGVRVYGNSHGLLASYAS
SRHSISCSVIGEGRNGEMERDYSYTVARHRDDLWTPESVGLQAAENTVSRLDPQKLKTGQ
FPVMFAADVATGLIGHLVMAISGGNLYRKSSFLLDHLGKTILPTWFNISERPHVMRGLAS
SPFDSEGVRTQDLEIITDGVLATYLLTSYAARKMNMTPTGHAGGIHNWYVKSTGQNYQQM
LKELGTGLLVTEVMGQGVNVVTGDYSRGAAGFWVENGEIKYPVSEITIAGNLKQMLQQIV
AVGSDIETRSQIQTGSILIESMKVAGE