Protein Info for CSW01_12605 in Vibrio cholerae E7946 ATCC 55056

Annotation: 3-isopropylmalate dehydratase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00170: 3-isopropylmalate dehydratase, large subunit
  • amino acids 4 to 467 (464 residues), 889.7 bits, E=1.9e-272
• PF00330: Aconitase
  • amino acids 9 to 459 (451 residues), 617.6 bits, E=8.6e-190

Best Hits

Swiss-Prot: 100% identical to LEUC_VIBCH: 3-isopropylmalate dehydratase large subunit (leuC) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 100% identity to vcj:VCD_001864)

MetaCyc: 83% identical to 3-isopropylmalate dehydratase subunit LeuC (Escherichia coli K-12 substr. MG1655)
3-isopropylmalate dehydratase. [EC: 4.2.1.33]

Predicted SEED Role

"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (4/8 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35

Protein Sequence (467 amino acids)

>CSW01_12605 3-isopropylmalate dehydratase large subunit (Vibrio cholerae E7946 ATCC 55056)
MSKAKTLYEKIYDAHVVVAAPGETPILYIDRHLVHEVTSPQAFDGLREKGRPVRQVSKTF
ATMDHNVSTTTKDINASGEMARIQMQTLSKNCEEFGVTLYDINHKYQGIVHVMGPELGIT
LPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQGRAKTMKIEVRGKVAPGI
TAKDIVLAIIGKTTAAGGTGYVVEFCGEAIRDLSMEGRMTVCNMAIELGAKAGLIAPDAT
TFNYIKGRKFAPQGSDWDAAVDYWQTLKTDEDAQFDAVVTLEASEIKPQVTWGTNPGQVI
AVDEPIPSPSQFADPVERSSAEKALAYMGLEAGKMLSDYKVDKVFVGSCTNSRIEDMRAA
AAVAKGKKVASHVQALIVPGSEQVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRL
GPGERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRQF