Protein Info for CSW01_12415 in Vibrio cholerae E7946 ATCC 55056

Annotation: two-component sensor histidine kinase BarA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 927 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 175 to 198 (24 residues), see Phobius details PF09984: sCache_4" amino acids 32 to 175 (144 residues), 175.1 bits, see alignment E=2.7e-55 PF18947: HAMP_2" amino acids 198 to 242 (45 residues), 28.1 bits, see alignment 5.6e-10 PF00672: HAMP" amino acids 198 to 246 (49 residues), 34.9 bits, see alignment 5.7e-12 PF00512: HisKA" amino acids 291 to 355 (65 residues), 77.8 bits, see alignment 1.8e-25 PF02518: HATPase_c" amino acids 402 to 517 (116 residues), 97.5 bits, see alignment E=2.4e-31 PF00072: Response_reg" amino acids 667 to 776 (110 residues), 99 bits, see alignment E=6.8e-32 PF01627: Hpt" amino acids 835 to 919 (85 residues), 46.2 bits, see alignment E=1.6e-15

Best Hits

KEGG orthology group: K07678, two-component system, NarL family, sensor histidine kinase BarA [EC: 2.7.13.3] (inferred from 100% identity to vcm:VCM66_2376)

Predicted SEED Role

"BarA sensory histidine kinase (= VarS = GacS)" in subsystem Type III secretion system orphans

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (927 amino acids)

>CSW01_12415 two-component sensor histidine kinase BarA (Vibrio cholerae E7946 ATCC 55056)
MTQRYGLRARVMTLTLAPTLIIGLLLSALFSFNRYHDLETQVINSGASIIEPLAIASEDA
LRMQSRESVRRIISYAHRKNSKLVRSIAVFDANHELFVTSNFHPNFEKLMYPKDKPIPFL
SSSVIDENTLILRTPIISERTVLDNGDANPATPVMGYIAIELDLSSLRLQQYQEIFSAGL
VLVIGILLSGVFASRLMYDVTRPITHMKNMVDRIRRGHLDVRIEGKMHGELDTLKKGINA
MAVSLSEYHVEMQHSIDQATSDLRETLEQLEIQNVELDIAKKRAQEAARVKSEFLANMSH
ELRTPLNGVIGFTRQMLKTQLTNSQADYLQTIEKSANNLLTIINDILDFSKLEAGKLALE
NIPFEFQEVLEEVVNLQATSAHEKGLEITLKIDPKIPRGVVGDPLRIQQVLTNLVGNSIK
FTERGNIDVSVEMRALRDDVIDLQFMVRDTGIGISERQQAQLFQAFSQADASISRRYGGT
GLGLVITQKLVSHMGGEISLTSRLHQGSTFWFTLRLHTTELPLNDGYNADSLNHRHLLLI
EPNMQAAAIVQQTLVQSGLEVTYRSAIPEEQHVYDYVLLNLAPSKETNPTLVELWVQRAL
AMTHNVIVGVPSTELALADQLMQRYPVKCISKPLSRKKLLQTLAAQQPQLANTSLPKPQA
DKLPLCVMAVDDNPANLKLITALLQERVEYVVSCTSGQEAIEQAQSRQFDIILMDIQMPH
MDGVTACKAIKQLKGYRDTPVIAVTAHAMAGERDRLLKAGMDDYLTKPIEEHILQQVLVH
WSPHTRSKQVAKVTPPDGAAVISNALPSSPPAEEAIIDWPVALRQSANKEDLAKEMLGML
VDYLREVETVVNTALEDEEYPASDLLHHIHKLHGSCSYSGVPRLRKICASLEQALRNGAS
IDELEPELFELQDEMAKVLEASRDYLN