Protein Info for CSW01_12345 in Vibrio cholerae E7946 ATCC 55056

Annotation: [glutamate--ammonia-ligase] adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 948 PF03710: GlnE" amino acids 33 to 280 (248 residues), 342.3 bits, see alignment E=1.7e-106 amino acids 557 to 809 (253 residues), 350.8 bits, see alignment E=4.2e-109 PF08335: GlnD_UR_UTase" amino acids 301 to 440 (140 residues), 117.1 bits, see alignment E=6.2e-38 amino acids 848 to 917 (70 residues), 22.1 bits, see alignment 1.4e-08

Best Hits

Swiss-Prot: 100% identical to GLNE_VIBCH: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 100% identity to vch:VC2438)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (948 amino acids)

>CSW01_12345 [glutamate--ammonia-ligase] adenylyltransferase (Vibrio cholerae E7946 ATCC 55056)
MSLPSALLPTAELHYQSLISEHPHIANWPSSVLNQLRYVLGLSQFVAQTLQRDDPLCQVL
PSLLAKPSREQYYRSELAQWLAECQDEAVAQKRLRQFRNQEMVYIAWRDFCASWTLEESL
SHLSQLAEALIFESYQWLYQRCCLEMGTPCNAQGEAQPMLIIGMGKLGGGELNFSSDIDL
IFTYPENGETQGARRSIANAQFFTRLGQRLIKLLDQSTPDGFCYRVDMRLRPFGDSGPLA
MSYAALEDYYQEQGRDWERYAMIKARVMGREMYPQYQELRQMLRPFVFRRYIDFSAIQSL
RRMKSMISSEVRRRGLSNNIKLGAGGIREVEFIAQVFQLIRGGREPSLRKRGLLETLDAI
AELELLTREQVQDLRDAYRFLRRLENLLQAMADKQTQTLPDKEDDQLRLSIAIGLADWPS
LQREVSEHMQRVHRVFATLIGEEDEEEEHTVARHFHELWDMAHKPEVIEHIIEQDLGLSD
AGEQIRTITQFKDDLAKRTIGPRGREVLNRLMPKVYQAVFAHPDAEFGLSRVLALLHSIA
TRTTYLELLDEHPAALVQLVRLCTASPMISEQLARYPILLDELIDPQHLYNPIPLESYQT
ELRDFLARIPEEDMEQQMEGLRQFKQISILRIAAADIAGVLPVMKVSDHLTYLAEAIVEA
VVSQAWLQVSSKYGEPTHLKHRDGRGFAVVGYGKVGGWELGYNSDLDIVFMHDCPVEVNT
DGEKSIDGRQFYLRLAQRIIHIFSTRTASGILYEVDTRLRPSGASGLLVSPTDAFDEYQR
QEAWTWEHQALVRARMIYGDAPLQQAFANTRHQILCLPREEHKLKQEVVEMRIKMRDHLG
GKKAGRFMLKQDEGGITDIEFLAQYLVLRFSHQQPKLTRWSDNVRIFESLMNHQVMSESQ
ALALTHAYTSMRDQIHRRNLLNQSADVRDSQFVVEREQVIQAWQQWLG