Protein Info for CSW01_12220 in Vibrio cholerae E7946 ATCC 55056
Annotation: pyruvate dehydrogenase (acetyl-transferring), homodimeric type
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 73% identical to ODP1_ECO57: Pyruvate dehydrogenase E1 component (aceE) from Escherichia coli O157:H7
KEGG orthology group: K00163, pyruvate dehydrogenase E1 component [EC: 1.2.4.1] (inferred from 87% identity to vsa:VSAL_I2625)MetaCyc: 73% identical to pyruvate dehydrogenase E1 component (Escherichia coli K-12 substr. MG1655)
Pyruvate dehydrogenase (acetyl-transferring). [EC: 1.2.1.104, 1.2.4.1]; 1.2.1.104 [EC: 1.2.1.104, 1.2.4.1]
Predicted SEED Role
"Pyruvate dehydrogenase E1 component (EC 1.2.4.1)" in subsystem Methionine Degradation or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.4.1)
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
- photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
- pyruvate fermentation to acetate II (3/3 steps found)
- pyruvate fermentation to acetate and (S)-lactate I (3/4 steps found)
- pyruvate fermentation to acetate V (2/3 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
- anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.2.1.104 or 1.2.4.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (886 amino acids)
>CSW01_12220 pyruvate dehydrogenase (acetyl-transferring), homodimeric type (Vibrio cholerae E7946 ATCC 55056) MSDMKHDVDALETQEWLAALESVVREEGVERAQYLLEQVLEKARLDGVDMPTGVTTNYIN TIPAAQEPAYPGDTTIERRIRSIIRWNAIMIVLRASKKDLELGGHMASFQSSAAFYETCF NHFFRAPNEKDGGDLVYYQGHISPGIYARAFVEGRLTEEQLDNFRQEVDGKGLPSYPHPK LMPEFWQFPTVSMGLGPISAIYQARFLKYLNGRGLKDTTAQRVYAFLGDGEMDEPESRGA ISFAAREKLDNLCFLINCNLQRLDGPVMGNGKIIQELEGLFRGAGWNVVKVIWGNGWDKL LAKDTTGKLLQLMNETIDGDYQTFKAKDGAYVREHFFGKYPETAALVADMTDDEIFALKR GGHESSKLYAAFKNAQDTKGRPTVILAKTVKGYGMGDAAEGKNIAHQVKKMDMTHVLAMR NRLGLQDLISDEEVKNLPYLKLEEGSKEFEYLHARRKALHGYTPQRLPNFTGEFIVPALE DFKPLLEEQSREISSTMAYVRSLNILLKDKNIGQNIVPIIADEARTFGMEGLFRQIGIYN PHGQNYTPQDRDIVSYYKEATSGQVLQEGINELGAMSSWVAAATSYSTNNLPMIPFYIYY SMFGFQRVGDMAWMAGDQQARGFLLGATAGRTTLNGEGLQHEDGHSHILAGTVPNCISYD PTFAYEVAVILQDGIRRMYGEQENVFYYLTLMNESYAHPAMPAGAEEGIRKGIYKLETHA GNKAKVQLMSSGTIMNEVRKAAQILSEEYGVASDVYSVTSFNELARDGQACDRFNMLHPE AEVKVPYIAQVMGTEPAIAATDYMKNYADQVRAFIPAQSYKVLGTDGFGRSDSRENLRRH FEVNAGYVVVAALNELAKRGEVEKSVVAAAIKKFDIDTEKTNPLYA