Protein Info for CSW01_12185 in Vibrio cholerae E7946 ATCC 55056

Annotation: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 512 TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 43 to 508 (466 residues), 601.8 bits, see alignment E=4.9e-185 PF01225: Mur_ligase" amino acids 44 to 122 (79 residues), 41.8 bits, see alignment E=1.7e-14 PF08245: Mur_ligase_M" amino acids 134 to 335 (202 residues), 179.4 bits, see alignment E=1.3e-56 PF02875: Mur_ligase_C" amino acids 358 to 484 (127 residues), 117.5 bits, see alignment E=1.1e-37

Best Hits

Swiss-Prot: 100% identical to MURE_VIBCH: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 100% identity to vcm:VCM66_2329)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (512 amino acids)

>CSW01_12185 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase (Vibrio cholerae E7946 ATCC 55056)
MLLMKTASKTNNSVRRNMNTHSAISELIAPWLELTDPKLAALVITHLELDSRLIKSGDTF
VAIQGHAVDGRQFIDKAIAQGANLVLAEADAQHLNGWVEYRAGVPVIYLAELGQHLSELA
GRLYGGHHNQLIGVTGTNGKTTITQLIAQWLELLGHKAAVMGTTGNGFLNALEPAANTTG
NALQIQATLRDLAERGAQYTALETSSHGLVQGRVKKLHFVAGVFSNLSRDHLDYHGTMEA
YAAAKFSLFSEHACQNAIINVDDAVGAQWVKQLPQAIGVSLVTKPNTAQAIWAREVAYAE
SGITLNFESSWGEGELHAPLIGEFNACNLLLALATLLSLGFEKSALLATAPKLRPVLGRM
ELFQREQKAKMVVDYAHTPDALEKALRALRVHCAGQLWAIVGCGGDRDRGKRPMMAAIAE
QFADRVILTDDNPRSESPQAIVADMVAGLTYPERAHIEHHRFQAASYALQHAGAQDIILL
AGKGHEDYQVLANETIHYSDRETAQQLLELQP