Protein Info for CSW01_10430 in Vibrio cholerae E7946 ATCC 55056
Annotation: 2-oxoglutarate dehydrogenase subunit E1
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 73% identical to ODO1_ECOL6: 2-oxoglutarate dehydrogenase E1 component (sucA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 82% identity to vsa:VSAL_I0846)MetaCyc: 73% identical to subunit of E1(0) component of 2-oxoglutarate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Oxoglutarate dehydrogenase (succinyl-transferring). [EC: 1.2.1.105, 1.2.4.2]
Predicted SEED Role
"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
- superpathway of glyoxylate bypass and TCA (10/12 steps found)
- 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
- TCA cycle I (prokaryotic) (8/10 steps found)
- TCA cycle III (animals) (8/10 steps found)
- TCA cycle VIII (Chlamydia) (5/6 steps found)
- TCA cycle II (plants and fungi) (7/9 steps found)
- L-tryptophan degradation III (eukaryotic) (3/15 steps found)
- superpathway of L-lysine degradation (9/43 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Lysine degradation
- Tryptophan metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.2.1.105 or 1.2.4.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (936 amino acids)
>CSW01_10430 2-oxoglutarate dehydrogenase subunit E1 (Vibrio cholerae E7946 ATCC 55056) MHNGVMKAWLESSHLAGANATYVEDLYELYLSDPDLVSEEWKRVFDGLPAHPTNVVEQPH SRVRDYFRRLAQETKHYNVQVSDPEVDAKQVKVLQLINAYRFRGHEAAKLDPLGLWNRPE VAELNPSFHNLTQEDMEETFNVGSFAIGKDTMKLKDIYQSLQKIYCGSVGAEYMHITDTE QKRWIQQRLEPVVGTPVFSKEEKRTFLEELTAAEGLERYLGAKFPGAKRFSLEGGDALVP MTKEMIRHAGASGMREVVIGMAHRGRLNMLVNVLGKKPQDLFDEFAGKHGEGWGTGDVKY HQGFSADFATPGGDVHLALAFNPSHLEIVNPVVMGSVRARQDRLGDEDGSKVLPITIHGD SAIAGQGVVAETFNMSQARGFCVGGTVRIVVNNQVGFTTSNPRDTRSTMYCTDIAKMVQA PIFHVNSDDPEAVAFVTRLALDYRNEFKRDVVIDLVCYRRHGHNEADEPNATQPLMYQKI KKHPTPRKLYADVLTERGECDLETATQLVNEYRDALDHGEVVVKEWRPMAMHSVDWSPYL GHDWHIPWNSEYAMERLQDLGRRVCQYPESHVLHSRVEKIYQDRLSMISGEKMLDWGMAE TLAYATLLDDGKRIRISGQDSGRGTFFHRHAVLHNQNDASTYIPLSQIHAGQGPFEVFDS VLSEEAVLAFEYGYATAEPSGLTLWEAQFGDFANGAQVVIDQFISSGEQKWGRLCGLTML LPHGYEGQGPEHSSARLERYLQLCAEQNMQVVVPSTPAQVYHMIRRQVVRPMRRPLVVMS PKSLLRHPLCVSSMEDLAHGTFQPAIGEIDALNPSQVKRVVFCSGKVYYDLLEQRRANEQ QDVAIVRIEQLYPFPLEEVQAAIANYTNVVDYVWCQEEPQNQGAWYSSQHNFRAAIPAGA DLKYAGRPASASPAVGYMSVHMKQQKALIEDALTLA