Protein Info for CSW01_09905 in Vibrio cholerae E7946 ATCC 55056

Annotation: long-chain-fatty-acid--CoA ligase FadD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 29 to 419 (391 residues), 308.7 bits, E=5.2e-96
• PF13193: AMP-binding_C
  • amino acids 470 to 544 (75 residues), 61.6 bits, E=1.1e-20

Best Hits

Swiss-Prot: 74% identical to LCFA_YERPE: Long-chain-fatty-acid--CoA ligase (fadD) from Yersinia pestis

KEGG orthology group: K01897, long-chain acyl-CoA synthetase [EC: 6.2.1.3] (inferred from 100% identity to vcj:VCD_002383)

MetaCyc: 72% identical to long-chain-fatty-acid--CoA ligase (Escherichia coli K-12 substr. MG1655)
Long-chain-fatty-acid--CoA ligase. [EC: 6.2.1.3]; 6.2.1.3 [EC: 6.2.1.3]; 6.2.1.3 [EC: 6.2.1.3]; 6.2.1.3 [EC: 6.2.1.3]; 6.2.1.3 [EC: 6.2.1.3]

Predicted SEED Role

"Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)" in subsystem Biotin biosynthesis or n-Phenylalkanoic acid degradation (EC 6.2.1.3)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• superpathway of fatty acids biosynthesis (E. coli) (40/53 steps found)
• long-chain fatty acid activation (1/1 steps found)
• palmitate biosynthesis III (21/29 steps found)
• phytol degradation (3/4 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (22/31 steps found)
• fatty acid salvage (4/6 steps found)
• stearate biosynthesis II (bacteria and plants) (4/6 steps found)
• stearate biosynthesis IV (4/6 steps found)
• γ-linolenate biosynthesis II (animals) (1/2 steps found)
• linoleate biosynthesis II (animals) (1/2 steps found)
• superpathway of fatty acid biosynthesis II (plant) (30/43 steps found)
• phosphatidylcholine acyl editing (2/4 steps found)
• alkane biosynthesis II (1/3 steps found)
• oleate biosynthesis I (plants) (1/3 steps found)
• 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
• octane oxidation (2/5 steps found)
• long chain fatty acid ester synthesis (engineered) (1/4 steps found)
• wax esters biosynthesis II (1/4 steps found)
• 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
• 2-deoxy-D-ribose degradation II (3/8 steps found)
• ceramide degradation by α-oxidation (2/7 steps found)
• sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
• stearate biosynthesis I (animals) (1/6 steps found)
• arachidonate biosynthesis III (6-desaturase, mammals) (1/7 steps found)
• capsaicin biosynthesis (1/7 steps found)
• icosapentaenoate biosynthesis II (6-desaturase, mammals) (1/7 steps found)
• icosapentaenoate biosynthesis III (8-desaturase, mammals) (1/7 steps found)
• ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
• sporopollenin precursors biosynthesis (4/18 steps found)
• cutin biosynthesis (1/16 steps found)
• suberin monomers biosynthesis (2/20 steps found)

KEGG Metabolic Maps

• Fatty acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.3

Use Curated BLAST to search for 6.2.1.3

Protein Sequence (566 amino acids)

>CSW01_09905 long-chain-fatty-acid--CoA ligase FadD (Vibrio cholerae E7946 ATCC 55056)
MDKPWLSRYPKDVPETINPDQYPSLVEMFEQSVHKYADQPAFMNMGAVMTFRKLEERSRA
FAAYLQNDLKLKKGDRVALMMPNLLQYPVALFGVLRAGMIAVNVNPLYTPRELEHQLNDA
DARAIVIVSNFANTLEQIVANTQVKHVVLTSLGQMLPRAKGTIVDFVVKYVKGMVPKYDL
PGAISMRKALHKGRRLQYVKPFMSGEDIAFLQYTGGTTGVAKGAILTHRNMVANVLQAKG
AYGPVLQEGRELVVTALPLYHVFALTVNCLLFIEMGGRNLLITNPRDIPGFVKELQKYPF
TAITGVNTLFNALVNNEDFHELDFKNMKLAVGGGMAVQRAVAERWKKTTGVHLLEGYGLT
ECSPLVTGNPYDLTDYTGAIGLPVPSTEVRIVDDAGNVVPNDQVGELQVRGPQVMQGYWQ
RPEATKEVLNAEGWLSTGDIVKFDDQGLIHIVDRKKDMILVSGFNVYPNEIEDVVALHGK
VLEVAAIGQANDVSGELVKIYVVKRDPSLTKDEVIAHCRKHLTGYKVPKLVEFRDDLPKT
NVGKILRRVLREENDAQLAAKAKQSA