Protein Info for CSW01_09610 in Vibrio cholerae E7946 ATCC 55056

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 426 TIGR00382: ATP-dependent Clp protease, ATP-binding subunit ClpX" amino acids 12 to 414 (403 residues), 695.7 bits, see alignment E=1.1e-213 PF06689: zf-C4_ClpX" amino acids 15 to 51 (37 residues), 72.3 bits, see alignment 8.4e-24 PF00493: MCM" amino acids 70 to 195 (126 residues), 25.1 bits, see alignment E=3e-09 PF01078: Mg_chelatase" amino acids 112 to 147 (36 residues), 21.1 bits, see alignment 6.2e-08 PF07724: AAA_2" amino acids 114 to 312 (199 residues), 117.6 bits, see alignment E=2.2e-37 PF07728: AAA_5" amino acids 116 to 193 (78 residues), 28.3 bits, see alignment E=5.8e-10 PF00004: AAA" amino acids 117 to 223 (107 residues), 57.3 bits, see alignment E=8.2e-19 PF10431: ClpB_D2-small" amino acids 319 to 396 (78 residues), 42.8 bits, see alignment E=1.6e-14

Best Hits

Swiss-Prot: 100% identical to CLPX_VIBCM: ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) from Vibrio cholerae serotype O1 (strain M66-2)

KEGG orthology group: K03544, ATP-dependent Clp protease ATP-binding subunit ClpX (inferred from 100% identity to vcj:VCD_002441)

MetaCyc: 84% identical to ATP-dependent Clp protease ATP-binding subunit ClpX (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpX" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (426 amino acids)

>CSW01_09610 ATP-dependent Clp protease ATP-binding subunit ClpX (Vibrio cholerae E7946 ATCC 55056)
MTDKSKEGGSSKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLCNDIIREEIKDVLPKK
ESAALPTPRKIREHLDDYVIGQEHAKKVLAVAVYNHYKRLRNGDTTSEGVELGKSNILLI
GPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVENIIQKLLQKCDYDVAKAER
GIVYIDEIDKISRKSENPSITRDVSGEGVQQALLKLIEGTVASVPPQGGRKHPQQEFLQV
DTSKILFICGGAFAGLDKVIEQRVATGTGIGFGADVRSKDNSKTLSELFTQVEPEDLVKY
GLIPEFIGRLPVTATLTELDEAALIQILCEPKNALTKQYAALFELENVDLEFREDALKAI
AAKAMKRKTGARGLRSILEAVLLETMYELPSMEEVSKVVIDESVINGESAPLLIYSANES
QAAGAE