Protein Info for CSW01_09410 in Vibrio cholerae E7946 ATCC 55056

Annotation: lipid A export ATP-binding/permease MsbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 582 transmembrane" amino acids 21 to 45 (25 residues), see Phobius details amino acids 66 to 93 (28 residues), see Phobius details amino acids 142 to 162 (21 residues), see Phobius details amino acids 168 to 186 (19 residues), see Phobius details amino acids 250 to 270 (21 residues), see Phobius details amino acids 280 to 298 (19 residues), see Phobius details TIGR02203: lipid A export permease/ATP-binding protein MsbA" amino acids 12 to 582 (571 residues), 824.2 bits, see alignment E=2.8e-252 PF00664: ABC_membrane" amino acids 28 to 296 (269 residues), 198 bits, see alignment E=4e-62 PF00005: ABC_tran" amino acids 359 to 509 (151 residues), 125.3 bits, see alignment E=4.2e-40

Best Hits

Swiss-Prot: 100% identical to MSBA_VIBCH: Lipid A export ATP-binding/permease protein MsbA (msbA) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K11085, ATP-binding cassette, subfamily B, bacterial MsbA [EC: 3.6.3.-] (inferred from 100% identity to vcj:VCD_002483)

MetaCyc: 68% identical to ATP-binding lipopolysaccharide transport protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-236 [EC: 7.5.2.6]; 7.5.2.6 [EC: 7.5.2.6]

Predicted SEED Role

"Lipid A export ATP-binding/permease protein MsbA" in subsystem ZZ gjo need homes

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.- or 7.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (582 amino acids)

>CSW01_09410 lipid A export ATP-binding/permease MsbA (Vibrio cholerae E7946 ATCC 55056)
MSLHSDESNWQTFKRLWTYIRLYKAGLVVSTIALVINAAADTYMISLLKPLLDEGFGNAE
SNFLRILPFMILGLMFVRGLSGFASSYCLSWVSGNVVMQMRRRLFNHFMHMPVRFFDQES
TGGLLSRITYDSEQVAGATSRALVSIVREGASIIGLLTLMFWNSWQLSLVLIVVAPVVAF
AISFVSKRFRKISRNMQTAMGHVTSSAEQMLKGHKVVLSYGGQEVERKRFDKVSNSMRQQ
TMKLVSAQSIADPVIQMIASLALFAVLFLASVDSIRAELTPGTFTVVFSAMFGLMRPLKA
LTSVTSEFQRGMAACQTLFGLMDLETERDNGKYEAERVNGEVDVKDVTFTYQGKEKPALS
HVSFSIPQGKTVALVGRSGSGKSTIANLFTRFYDVDSGSICLDGHDVRDYKLTNLRRHFA
LVSQNVHLFNDTIANNIAYAAEGEYTREQIEQAARQAHAMEFIENMPQGLDTVIGENGTS
LSGGQRQRVAIARALLRDAPVLILDEATSALDTESERAIQAALDELQKNKTVLVIAHRLS
TIEQADEILVVDEGEIIERGRHADLLAQDGAYAQLHRIQFGE