Protein Info for CSW01_09290 in Vibrio cholerae E7946 ATCC 55056

Annotation: ATP-dependent DNA helicase DinG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 691 PF06733: DEAD_2" amino acids 198 to 262 (65 residues), 23.5 bits, see alignment E=4.1e-09 PF13307: Helicase_C_2" amino acids 526 to 684 (159 residues), 151.2 bits, see alignment E=2.9e-48

Best Hits

Swiss-Prot: 62% identical to DING_PHOPR: ATP-dependent DNA helicase DinG (dinG) from Photobacterium profundum (strain SS9)

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 100% identity to vcm:VCM66_1778)

Predicted SEED Role

"ATP-dependent helicase DinG/Rad3" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (691 amino acids)

>CSW01_09290 ATP-dependent DNA helicase DinG (Vibrio cholerae E7946 ATCC 55056)
MLTKNIQDSIRNSYQNLQNQLENFIPRRAQNFLVAEMAKTLCGAYHKSTQMLVAEAGTGI
GKSLSYLMAVIPVAVVNKRKVVISTATVALQEQLLNKDLPLYRRISDQNFSFILAKGRQR
YCCSERLAAACGIDDGQIALFESKPKPHETELLAELHTALSQGKWDGDRDGWPSPISDEL
WSVIVSDKHSCNGSFSVHRHCPFQKARSELDKADVIIANHSLVMADADLGGGVILPAPEE
TIYVFDEAHHLPTVAREHASAAATLKGAASWLEKLNQSLSKFTALGDEKRAERFAEEART
AIQYLIPTLNQLPKQFMAEQFVEGIYRFEHGELPQWLADESQALSKSSQKAMQSVSKVAD
LIAEKVKEGELATRIAEPALGELGFYVQRLENLTQVWQLMAKPNKDKGAPLARWLEVSPE
REGDYLVSVSPLEIGWQLDQQLWSRCVGAILVSATLRALNSFGFFCRQAGISDKAEDGVQ
FLALASPFDYPTQGELLIPKMEMEPQAEGYTAYLAKKVLCYLQADKANLVLFASYWQMRE
VAESLKVEFTKRGWALQVQGEKSRSEILNKHKKLIEKQKTSVLFGTGSFSEGLDLPGELL
ENLIITKIPFAVPTSPVEQAHAEYIQELGGNPFMQITVPEASKKLIQSVGRLLRKERDSG
RVVILDRRVVSKRYGKALLDALPPFKRTIEY