Protein Info for CSW01_09255 in Vibrio cholerae E7946 ATCC 55056

Annotation: cysteine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 459 TIGR00435: cysteine--tRNA ligase" amino acids 2 to 458 (457 residues), 640.5 bits, see alignment E=9.8e-197 PF01406: tRNA-synt_1e" amino acids 15 to 313 (299 residues), 489.4 bits, see alignment E=1.3e-150 PF09334: tRNA-synt_1g" amino acids 36 to 107 (72 residues), 27.3 bits, see alignment E=4.8e-10 amino acids 244 to 310 (67 residues), 28.2 bits, see alignment E=2.5e-10 PF09190: DALR_2" amino acids 341 to 394 (54 residues), 77.7 bits, see alignment 2.3e-25 PF23493: CysS_C" amino acids 412 to 457 (46 residues), 32.6 bits, see alignment 2.1e-11

Best Hits

Swiss-Prot: 100% identical to SYC_VIBC3: Cysteine--tRNA ligase (cysS) from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 100% identity to vco:VC0395_A1439)

MetaCyc: 75% identical to cysteine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (459 amino acids)

>CSW01_09255 cysteine--tRNA ligase (Vibrio cholerae E7946 ATCC 55056)
MLKIYNSLTRQKEEFKPIVAGKVGMYVCGVTIYDLCHIGHGRTFVSFDVVARYLRYLGYD
LTFVRNITDIDDKIIKRAAENAETCESLTERLIQEMYADFDALNIKRPDVEPRATAYIEE
IIALVERLIERGFAYVADNGDVMFEVSQYSEYGKLSKQDLDQLQAGARVDIEAAKRSPLD
FVLWKMSKPGEPTWESPWGSGRPGWHIECSAMNSSILGTHFDIHGGGSDLQFPHHENEIA
QSCCAHDTQYVNTWMHSGMVMVDKEKMSKSLGNFFTIRDVLGHYDAETVRYFLMSGHYRS
QLNYSEENLNQARASLERLYNALRGLDRSVPAAGGEEYVTRFTAAMNDDFNTPEAYSVLF
DMAREINRLKSEDMTNASALGSLMRELADVIGILYQEPEAFFQGSAEDEDAAQIEALIKL
RNDSRATKDWANADLARDKLNEMGIVLEDGPNGTTWRRK