Protein Info for CSW01_08575 in Vibrio cholerae E7946 ATCC 55056

Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 4 to 314 (311 residues), 402.5 bits, E=1.6e-124
  • amino acids 549 to 689 (141 residues), 31.5 bits, E=1.4e-11
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 7 to 758 (752 residues), 1151.8 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 156 to 328 (173 residues), 23.8 bits, E=2.7e-09
  • amino acids 431 to 753 (323 residues), 508.1 bits, E=1.1e-156

Best Hits

Swiss-Prot: 100% identical to METE_VIBCH: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 100% identity to vcm:VCM66_1644)

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• S-adenosyl-L-methionine salvage I (4/4 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• S-adenosyl-L-methionine salvage II (2/3 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• L-methionine biosynthesis III (2/4 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Protein Sequence (761 amino acids)

>CSW01_08575 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (Vibrio cholerae E7946 ATCC 55056)
MTTTHILGYPRIGEKRELKFALEKYWRGEIDQAALKQVGSQIRQKNWALQKEAGLDFVTA
GDFAWYDHVLTTTLLLGHVPKRHSHGFPDLDTLFRVGRGQSQNACGCGTGSAASDMTKWF
NTNYHYIVPEFSSNDTFNVSWPQLFEEVNEALQAGHDVKPVLLGPISYLYLGKEVEEGFD
RLTLLPRLLTAYQAILSKLAKQGVQWVQIDEPILALELEPRWQEAFKLAYQVIRGDVKLL
LTTYFDSVLDTLDKIVELPVDGLHVDISAAPAQLETIVNRLPSDWVLSAGVINGRNVWRA
DLSAILARLQPVKTLLGERLWVASSCSLLHSPVDLDLEGDLSAETRSWFAFAKQKVTEVA
LLGRALEGDAAAILACDTYSQPIVARKSSHIVNKASVQTRINNITAALAERSAPYIERAH
HQAEVLGLPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEAL
GLDVLVHGEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVE
WSTYAQSLTSKQMKGMLTGPVTILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINI
IQIDEPAIREGLPLKKRDHQTYLDWAVQAFKISAGSARPETQIHTHMCYSEFNEIIESVA
ALDADVITIETSRSNMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQAWIEDLLRKAAEKI
PAQRLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAEWQA