Protein Info for CSW01_08305 in Vibrio cholerae E7946 ATCC 55056

Annotation: diguanylate phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 584 PF00072: Response_reg" amino acids 3 to 117 (115 residues), 64.1 bits, see alignment E=2.6e-21 PF00563: EAL" amino acids 141 to 370 (230 residues), 237.6 bits, see alignment E=2.6e-74 PF00196: GerE" amino acids 526 to 580 (55 residues), 68.9 bits, see alignment 4.6e-23

Best Hits

KEGG orthology group: K13246, c-di-GMP phosphodiesterase [EC: 3.1.4.52] (inferred from 100% identity to vco:VC0395_A1258)

Predicted SEED Role

"Response regulator VieA"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.4.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (584 amino acids)

>CSW01_08305 diguanylate phosphodiesterase (Vibrio cholerae E7946 ATCC 55056)
MKIMIVEDDRIQATSLKLKLRQLGLDDVILAENGFAALELCNKVGIDLMFCDIRMPQMDG
ISLLSQLSLQAPKLGVVILSAVEDTILELTHNMCSLAGFAYVDRLPKPYEVEDLQRVVEG
FQKQTEPETSHKPAITLTCDEIEQAFLHDHIFNYYQPQFDFRSGAMVGVEALVRYEHPTH
GMLSPAVFLPLIEQCGLHEKLFLTVLEKSVSALASIGADLQLSVNISQRNLQHSICDPIL
AICERYGFPASKLTLEMTEHEVYNGTPTSLANLARLRMYGVGLSIDDFGTGYASLGQLAQ
LPFTELKIDRSFVHDLATNYKHQQLTNMCLLLAQSLGLHCVVEGVENEETWQYLRQLGVD
TCQGYYAAKPMPIAQLSELYTKNRRQCLGCQSMDDGWQILLVDNHMLSATALQKVLNKEE
NVSKVFVAQDIEQTLHKLRDLPINLLVVESRFLSTISPAAFHELLEPYYRGALLVLHDGL
TAEAQEDEPKAIVVKANTLMETSSRILLQAQQSNVRPQAQQEELAQLSERELTVAKMLVE
GLTNKEIALQLDINQKTVSTYKTRIMTKLGIKSAMELVRFVTLK