Protein Info for CSW01_07180 in Vibrio cholerae E7946 ATCC 55056

Annotation: tRNA 2-thiocytidine(32) synthetase TtcA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 PF01171: ATP_bind_3" amino acids 43 to 203 (161 residues), 58 bits, see alignment E=5.3e-20

Best Hits

Swiss-Prot: 100% identical to TTCA_VIBCH: tRNA-cytidine(32) 2-sulfurtransferase (ttcA) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K14058, tRNA 2-thiocytidine biosynthesis protein TtcA (inferred from 99% identity to vco:VC0395_A1043)

MetaCyc: 79% identical to tRNA cytosine32 2-sulfurtransferase TtcA (Escherichia coli K-12 substr. MG1655)
2.8.1.M2 [EC: 2.8.1.M2]

Predicted SEED Role

"tRNA(Cytosine32)-2-thiocytidine synthetase"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (310 amino acids)

>CSW01_07180 tRNA 2-thiocytidine(32) synthetase TtcA (Vibrio cholerae E7946 ATCC 55056)
MTAQTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDIL
MSLQKSAPISFELVAVNLDQKQPGFPEHVLPEYLESLGVEYKIVEEDTYAIVQDKVPEGK
TTCALCSRLRRGILYRTAKELGATKIALGHHRDDILETLFLNMFYGGKMKGMPPKLVSDN
GEHVVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRI
ETMFRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVVEDEDLVMEFD
PSLQLNVTNI