Protein Info for CSW01_06860 in Vibrio cholerae E7946 ATCC 55056
Annotation: siroheme synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02304, precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 4.99.1.4] (inferred from 100% identity to vcm:VCM66_1318)Predicted SEED Role
"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.3.1.76, EC 4.99.1.4)
MetaCyc Pathways
- siroheme biosynthesis (4/4 steps found)
- factor 430 biosynthesis (3/7 steps found)
- cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (3/15 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (18/36 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.3.1.76 or 4.99.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (312 amino acids)
>CSW01_06860 siroheme synthase (Vibrio cholerae E7946 ATCC 55056) MRYFPLFLDLTNKPVLVVGGGEVACRKIDALLRADAKVTVISPQVAPALQAWIEQGKCHW IQHFYSSHWLDKRYVQVWATTDNPELNHQVYKDAKEQGILVNVVDDQPYCDFITPSMIER GRIQLAISSGGASPVLIRNIRETLEAVLAQNLALLADFGASKRNSIKDFLPSVDLRRQFW ERFFAHPEVKNAQDRESLERIYIHLLTQSTDKVSATTWIEFGADVELLSLKALRYMQQAE LVLHTQDCPFVFVDLCRRDAQRQSFNSSVELSTLLLQAQQETQNVCVLIPSGSSEYALLQ GKATVLKMAQQG