Protein Info for CSW01_06740 in Vibrio cholerae E7946 ATCC 55056

Annotation: 2-methylcitrate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 TIGR01800: 2-methylcitrate synthase/citrate synthase II" amino acids 11 to 376 (366 residues), 548.5 bits, see alignment E=3e-169 PF00285: Citrate_synt" amino acids 11 to 359 (349 residues), 413 bits, see alignment E=5.3e-128

Best Hits

Swiss-Prot: 69% identical to PRPC_SHEON: 2-methylcitrate synthase (prpC) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01659, 2-methylcitrate synthase [EC: 2.3.3.5] (inferred from 100% identity to vcm:VCM66_1292)

MetaCyc: 52% identical to 2-methylcitrate synthase (Escherichia coli K-12 substr. MG1655)
2-methylcitrate synthase. [EC: 2.3.3.5]

Predicted SEED Role

"2-methylcitrate synthase (EC 2.3.3.5)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 2.3.3.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (377 amino acids)

>CSW01_06740 2-methylcitrate synthase (Vibrio cholerae E7946 ATCC 55056)
MSAAKELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLAHHAQFEEVAHLLLVGYL
PTQAELDQYKTRLIGLRGLPEKLKQALELIPAEAHPMDVMRTGCSILGNLEPEHTFAEQQ
AATERMLALFPAIICYWYRFSHEGVRIDTADQSEDSIGGYFLKMLTGQAPSELFKKVMHC
SLTLYAEHEFNASTFAARVCASTLSDIHSCVTGAIGTLRGPLHGGANEAAMAMIEQWHSA
DEAEAGIMRMLASKEKIMGFGHAIYRESDPRNALIKEWSKALSEAVGDSHLYAVSERVEA
VMKREKDLFCNADFFHASAYHFMGIPTKLFTPIFVMSRLTGWAAHVYEQRANNRIIRPSA
DYVGPEHQTWLPIEQRG