Protein Info for CSW01_06355 in Vibrio cholerae E7946 ATCC 55056

Annotation: DNA topoisomerase (ATP-hydrolyzing) subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 894 TIGR01063: DNA gyrase, A subunit" amino acids 8 to 863 (856 residues), 1247.9 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 506 (475 residues), 558.5 bits, see alignment E=1e-171 PF03989: DNA_gyraseA_C" amino acids 537 to 584 (48 residues), 36.9 bits, see alignment 2e-13 amino acids 587 to 636 (50 residues), 42.9 bits, see alignment 2.6e-15 amino acids 644 to 687 (44 residues), 34 bits, see alignment 1.6e-12 amino acids 692 to 709 (18 residues), 11.1 bits, see alignment (E = 2.3e-05) amino acids 734 to 761 (28 residues), 26.4 bits, see alignment (E = 3.7e-10) amino acids 767 to 813 (47 residues), 50.9 bits, see alignment 8e-18 amino acids 817 to 863 (47 residues), 45.5 bits, see alignment 4.1e-16

Best Hits

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (894 amino acids)

>CSW01_06355 DNA topoisomerase (ATP-hydrolyzing) subunit A (Vibrio cholerae E7946 ATCC 55056)
MSDLAKEITPVNIEDELRSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMNVLGNDWN
KAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAM
RYTEVRMSKIAHELLADLDKETVDYVPNYDGTEQIPAVLPTKIPNLLINGASGIAVGMAT
NIPPHNLGEVIDGCLAYIDNEAITIDELMDYIPGPDFPTAALISGRKGIIDAYKTGRGKV
YMRSKAEIETDKNGKETIIVSEIPYQVNKARLIEKIAELVKEKKVEGISALRDESDKDGM
RIVIECKRDAVGEVVLNNLYANTQLQTTFGINMVALDNGQPKLFNLKEMLKCFVDHRREV
VTRRTIFELRKARERAHILEGLALALANIDEIIELIRRAATPAEAKEGLIARGWDLGNVA
AMLERAGTDAARPEWLEPQYGIRDGKYFLTEQQAQAILDLRLHKLTGLEHEKILDEYKQL
LEEIAALMHILASTERLMEVIREELEAIRAGFSDARRTEITAATHDIDMEELIAREDVVV
TLSHEGYVKYQLLSDYEAQRRGGKGKSATKMKEEDYIERLLVANTHDNILLFSTRGKTYR
MKVYQLPLASRTARGKPIVNLLPLEENERITAILPVTEFSEDKFIFMATGDGTVKKTSLD
QFANVRANGLIALNLRDEDSLIGVDITDGESEIMLFSKFGKVVRFKESEETAVVDENGQP
VLDENGQPEIKFKGVRPMGRTAAGVRGMKLADGDQVVSLIVPKTEGDVLTVTENGYGKRT
SLSEYPTKGRGTQGVVSIKVSERNGSVVGAVQVAEGDEFMMITNAGTLVRTRVAEVSQVG
RNTQGVTLIRTSEGESVVGLQRIDEIEESELPEGEEALVENDAPVAQDDDGEQE