Protein Info for CSW01_05760 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 TIGR01261: histidinol-phosphatase" amino acids 5 to 164 (160 residues), 260.6 bits, see alignment E=5.7e-82 PF08645: PNK3P" amino acids 6 to 127 (122 residues), 26.9 bits, see alignment E=5.4e-10 TIGR01656: histidinol-phosphate phosphatase domain" amino acids 6 to 147 (142 residues), 154.2 bits, see alignment E=3.5e-49 TIGR01662: HAD hydrolase, family IIIA" amino acids 6 to 151 (146 residues), 94.4 bits, see alignment E=9.8e-31 PF00475: IGPD" amino acids 198 to 339 (142 residues), 213.6 bits, see alignment E=1.9e-67

Best Hits

Swiss-Prot: 100% identical to HIS7_VIBCH: Histidine biosynthesis bifunctional protein HisB (hisB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01089, imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase [EC: 3.1.3.15 4.2.1.19] (inferred from 100% identity to vco:VC0395_A0705)

MetaCyc: 69% identical to imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase (Escherichia coli K-12 substr. MG1655)
Imidazoleglycerol-phosphate dehydratase. [EC: 4.2.1.19]; Histidinol-phosphatase. [EC: 4.2.1.19, 3.1.3.15]

Predicted SEED Role

"Histidinol-phosphatase (EC 3.1.3.15) / Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)" in subsystem Histidine Biosynthesis (EC 3.1.3.15, EC 4.2.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.15 or 4.2.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (357 amino acids)

>CSW01_05760 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase (Vibrio cholerae E7946 ATCC 55056)
MSKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKLEPYVIPSLLKLQEAGYRLVMVTNQDG
LGTSSYPQADFDAPHNMMMDIFESQGVKFNDVLICPHFERDNCSCRKPKLGLVKEYLQGG
KVDFKSSAVIGDRMTDLQLAENMAIRGIQYHPQTMGWLDIVKDLTTKPRVAQVVRKTKET
DIQVLVNLDQTGGNQIETGLGFFDHMLDQIATHGGFQLQLKVVGDLHIDDHHTVEDTALA
LGQALREALGDKRGIGRFGFTLPMDECLAQCALDLSGRPYLKFDASFSRPQVGDLSTEMV
YHFFRSLTDTLACTLHLSSSGDNDHHIIESLFKAFGRTLRQAITVQGNDLPSSKGVL