Protein Info for CSW01_05265 in Vibrio cholerae E7946 ATCC 55056

Annotation: methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 683 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR00398: methionine--tRNA ligase" amino acids 9 to 535 (527 residues), 663.6 bits, see alignment E=2.3e-203 PF09334: tRNA-synt_1g" amino acids 9 to 396 (388 residues), 548.4 bits, see alignment E=2.5e-168 PF00133: tRNA-synt_1" amino acids 11 to 81 (71 residues), 39.1 bits, see alignment E=1.3e-13 PF19303: Anticodon_3" amino acids 413 to 541 (129 residues), 38.4 bits, see alignment E=3.4e-13 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 542 to 682 (141 residues), 158.9 bits, see alignment E=6.4e-51 PF01588: tRNA_bind" amino acids 588 to 680 (93 residues), 94.3 bits, see alignment E=1.2e-30

Best Hits

Swiss-Prot: 100% identical to SYM_VIBCH: Methionine--tRNA ligase (metG) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 100% identity to vco:VC0395_A0554)

MetaCyc: 73% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (683 amino acids)

>CSW01_05265 methionine--tRNA ligase (Vibrio cholerae E7946 ATCC 55056)
MANDPRKLLVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNTVNFICADDAHGTPIM
LKAQQMGMTPEAMIEMVSEEHQRDFAGFDISFDNYHSTHSDENRELASHIYLQLKKNGFI
SSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEDQYGDNCDACGETYSPTELINPKSAVSG
ATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQSETANKMQEWFEGGLQQWDISRDAPYFG
FEIPGEKDKFFYVWLDAPIGYMGSFKNLCDKRGDLDFNEYWNKDSKTELYHFIGKDIVYF
HSLFWPAMLDGSGFRKPTNVFVHGYVTVNGAKMSKSKGTFVKASTYLNHLDPECLRYYYA
AKLNNRIDDLDLNLEDFTQRVNADVVNKIVNLASRNAGFITKRFDGKLSAHFAEPELYAE
FAGAADRIAELFEAREFGRAIREITALADKANQYVDEKAPWVVAKQEGQDQALQDICTVG
INLFRVLMTYLKPVMPALAERTEAFLNQELTWEGVATPLTDHAVTPFKALFNRIDPKQVE
AMIEASKAEAAAEKAAADAAKPKSAETELSKDPLAAEIEFDDFAKVDLRIAKILSCEAVE
KSDKLLKFELDIGGETRQVFSGIKSAYQPEDLIGKYTVVVANLKPRKMKFGMSEGMILAA
GPGGSDLWLLEPHQGAQAGMRVM