Protein Info for CSW01_04930 in Vibrio cholerae E7946 ATCC 55056

Annotation: phosphoenolpyruvate-protein phosphotransferase PtsI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 573 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 2 to 565 (564 residues), 826.1 bits, see alignment E=6.6e-253 PF05524: PEP-utilisers_N" amino acids 4 to 126 (123 residues), 120.4 bits, see alignment E=7.4e-39 PF00391: PEP-utilizers" amino acids 153 to 223 (71 residues), 76.9 bits, see alignment E=1.1e-25 PF02896: PEP-utilizers_C" amino acids 250 to 541 (292 residues), 430.7 bits, see alignment E=4e-133

Best Hits

Swiss-Prot: 75% identical to PT1_SALTY: Phosphoenolpyruvate-protein phosphotransferase (ptsI) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] (inferred from 100% identity to vcj:VCD_003372)

MetaCyc: 75% identical to PTS enzyme I (Escherichia coli K-12 substr. MG1655)
Phosphoenolpyruvate--protein phosphotransferase. [EC: 2.7.3.9]

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (573 amino acids)

>CSW01_04930 phosphoenolpyruvate-protein phosphotransferase PtsI (Vibrio cholerae E7946 ATCC 55056)
MISGILASPGIAIGKALLLQEDEIVLNTNTISDDQVEAEVERFYTARDKSSAQLEVIKQK
ALETFGEEKEAIFEGHIMLLEDEELEEEILALIKKEKMSADNAIHTVIEEQATALESLDD
EYLKERATDIRDIGTRFVKNALGMHIVSLSEIDQEVVLVAYDLTPSETAQINLNYVLGFA
CDIGGRTSHTSIMARSLELPAIVGTNDITKKVKNGDTLILDAMNNKIIVNPTQAQIEEAK
AVKAAFLAEKEELAKLKDLPAETLDGHRVEVCGNIGTVKDCDGIIRNGGEGVGLYRTEFL
FMDRDALPTEEEQYQAYKEVAEAMNGQAVIIRTMDIGGDKDLPYMDLPKEMNPFLGWRAV
RISLDRREILRDQLRGILRASAHGKLRVMFPMIISVEEIRELKNAIEEYKAELRTEGHAF
DENIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
VLTVIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNA
NYAEIKAMAEEALALPTAAEIEACVDKFIAAKA