Protein Info for CSW01_04885 in Vibrio cholerae E7946 ATCC 55056

Annotation: leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 858 TIGR00396: leucine--tRNA ligase" amino acids 5 to 857 (853 residues), 1249.4 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 224 (211 residues), 104.8 bits, see alignment E=9.1e-34 amino acids 417 to 571 (155 residues), 35.8 bits, see alignment E=8.6e-13 amino acids 618 to 651 (34 residues), 30.2 bits, see alignment (E = 4.1e-11) PF09334: tRNA-synt_1g" amino acids 39 to 180 (142 residues), 77.7 bits, see alignment E=1.6e-25 PF13603: tRNA-synt_1_2" amino acids 221 to 404 (184 residues), 270.1 bits, see alignment E=1.6e-84 PF08264: Anticodon_1" amino acids 698 to 819 (122 residues), 66.6 bits, see alignment E=4.7e-22

Best Hits

Swiss-Prot: 88% identical to SYL_ALIF1: Leucine--tRNA ligase (leuS) from Aliivibrio fischeri (strain ATCC 700601 / ES114)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 86% identity to vsa:VSAL_I0990)

MetaCyc: 75% identical to leucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Leucine--tRNA ligase. [EC: 6.1.1.4]; 6.1.1.4 [EC: 6.1.1.4]; 3.1.1.- [EC: 6.1.1.4]

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (858 amino acids)

>CSW01_04885 leucine--tRNA ligase (Vibrio cholerae E7946 ATCC 55056)
MQEQYNPQDIEHKVQQHWDNNKTFVVSEDPNKEKFYCLSMFPYPSGRLHMGHVRNYTIGD
VVSRFQRLQGKNVLQPIGWDAFGLPAENAAVKNNTAPAPWTYENIEYMKNQLKLLGFGYD
WNREFATCRPEYYRWEQEFFTKLFAKGLVYKKTSSVNWCPNDQTVLANEQVEDGCCWRCD
TPVEQKEIPQWFIKITAYAQELLDDLDNLDGWPEMVKTMQRNWIGRSEGVELKFAVKGEN
TDLEVYTTRPDTLMGVTYVGIAAGHPLATKAAANNPALAAFIDECKNTKVAEAEIATMEK
KGMATGLTAIHPLNGREVPIYIANFVLMDYGTGAVMAVPAHDQRDFEFATKYGLDIIPVI
KPADGSELDVSEAAYTEKGVLFASGEFDGLDFQAAFNAIAAKLEAEGKGKKTVNFRLRDW
GVSRQRYWGAPIPMVTTEDGQVHPVPADQLPVILPEDVVMDGVTSPIKADKEWAKTTFNG
EPALRETDTFDTFMESSWYYARYCSPQADDILDPEKANYWLPVDQYIGGIEHACMHLLYS
RFFHKLLRDAGYVKSDEPFKKLLCQGMVLADAFYYTNDKGGKEWVSPTEVKVERDGKGRI
VSAVDATGRQVEHSGMIKMSKSKNNGIDPQEMVDKYGADTVRLFMMFASPADMTLEWQES
GVEGANRFLRRVWKLVREHTELGQAPALDASALNADQKALRRDVHKTIAKVTDDVARRQT
FNTAIAAIMELMNKLTKAPMTEAQDRAILDEALKAITLMLYPITPHICFEMWVALGQSNI
DTASWPTYDEAALVEDEKLIVLQVNGKLRGKLTVAADATQQQVEALGMQDENVQKFIDGL
TVRKVIYVPGKLLNIVAN