Protein Info for CSW01_04500 in Vibrio cholerae E7946 ATCC 55056

Annotation: proline--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 567 (567 residues), 818.2 bits, E=2.3e-250
• PF00587: tRNA-synt_2b
  • amino acids 96 to 459 (364 residues), 108.4 bits, E=6.8e-35
• PF04073: tRNA_edit
  • amino acids 257 to 375 (119 residues), 88.2 bits, E=7e-29
• PF03129: HGTP_anticodon
  • amino acids 475 to 568 (94 residues), 85.5 bits, E=3.6e-28

Best Hits

Swiss-Prot: 100% identical to SYP_VIBC3: Proline--tRNA ligase (proS) from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 100% identity to vch:VC0875)

MetaCyc: 72% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (21/21 steps found)
• anguibactin biosynthesis (2/8 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• pyochelin biosynthesis (1/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Protein Sequence (571 amino acids)

>CSW01_04500 proline--tRNA ligase (Vibrio cholerae E7946 ATCC 55056)
MRTSNYLLSTLKETPNDAEVVSHKLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQE
IDNAGAIETLMPVVQPFELWEETGRSEKMGPELLRFTDRHERPFVLSPTAEEVITALVRN
EVSSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDAYSFDIDKAGLEKSYEAMHVA
YCKAFDRMGLDYRPVLADTGAIGGNGSHEFHVLAESGEDLIAFSTESDYAANIEKAEAVA
PAIERPAPTQAMTLVDTPNAKTIAELVEQHGLPIEKTVKTLFVKASDEIDAPIVALIIRG
DHELNEIKAEKLAEVASPLEMASEEEIRALIGAGPGSLGPVGLKLPFIVDRTVAVMNDFG
AGANIDGKHYFGINWGRDVELGKVEDLRNVVEGDPSPCGKGTLMLKRGIEVGHIFQLGTN
YSEKMNCGVLDSNGKNVILEMGCYGIGVSRVVAAAIEQNHDDYGIIWPDAIAPFQVAIVP
MNMHKSERVQQAAEKLYAELTAAGIEVLFDDRKERPGVMFSDMELIGVPHTIIIGDRSMD
EGHFEYKNRRQGEKEAVAMESIIDFIQAKLA