Protein Info for CSW01_04125 in Vibrio cholerae E7946 ATCC 55056

Annotation: citrate (pro-3S)-lyase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details TIGR01588: citrate (pro-3S)-lyase, beta subunit" amino acids 8 to 290 (283 residues), 465.9 bits, see alignment E=2e-144 PF03328: HpcH_HpaI" amino acids 11 to 230 (220 residues), 306.1 bits, see alignment E=1.2e-95 PF15617: C-C_Bond_Lyase" amino acids 14 to 290 (277 residues), 29.7 bits, see alignment E=3.8e-11

Best Hits

Swiss-Prot: 78% identical to CITE_ECOLI: Citrate lyase subunit beta (citE) from Escherichia coli (strain K12)

KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 100% identity to vcm:VCM66_0756)

MetaCyc: 78% identical to citrate lyase beta subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Citrate lyase beta chain (EC 4.1.3.6)" (EC 4.1.3.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.6

Use Curated BLAST to search for 4.1.3.34 or 4.1.3.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (296 amino acids)

>CSW01_04125 citrate (pro-3S)-lyase subunit beta (Vibrio cholerae E7946 ATCC 55056)
MEPAIMTKLRRSMLFVPGANAAMLSNTFIYQPDSIMFDLEDSVALREKDTARMLVFHALQ
HPMYQEMETVVRVNPLDSEFGIKDLNAVVRGGAKVVRLPKTDNANDVIEMEKVIEQIERA
CGREVGSTRMLAAIESAQGINNAVEIAFSSRRLIGIALGAEDYVRDLRTQRSAEGIELLF
ARCSILQAARAAGIMAFDTVYSDANNEEGFLREAEHIKQLGFDGKSLINPRQIDLLHNVF
APTQKEVDHAIAVIEAAEEAAAKGLGVVSLNGKMVDGPIIERARWTLQRAESGIKH