Protein Info for CSW01_04005 in Vibrio cholerae E7946 ATCC 55056

Annotation: 2,3-dihydroxybenzoate-AMP ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 543 TIGR02275: (2,3-dihydroxybenzoyl)adenylate synthase" amino acids 3 to 531 (529 residues), 941.7 bits, see alignment E=4.4e-288 PF00501: AMP-binding" amino acids 31 to 397 (367 residues), 243.9 bits, see alignment E=2.6e-76 PF13193: AMP-binding_C" amino acids 448 to 524 (77 residues), 53.4 bits, see alignment E=4.2e-18

Best Hits

Swiss-Prot: 100% identical to VIBE_VIBCH: Vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase (vibE) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K04776, vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase [EC: 2.7.7.58] (inferred from 100% identity to vco:VC0395_A0301)

MetaCyc: 100% identical to (2,3-dihydroxybenzoyl)adenylate synthase (Vibrio cholerae)
DHBAMPLIG-RXN [EC: 6.2.1.71]; 6.2.1.71 [EC: 6.2.1.71]; RXN-10988 [EC: 6.2.1.71]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.58 or 6.2.1.71

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (543 amino acids)

>CSW01_04005 2,3-dihydroxybenzoate-AMP ligase (Vibrio cholerae E7946 ATCC 55056)
MTTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERI
EQLAAGFTELGLGCGDNVVLQLGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQH
SQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQTRVTDPKFIELASCYSASSCQ
ANADPNQIAFFQLSGGTTGTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNF
PLSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYD
LSSLHFVQVGGAKFSEAAARRLPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRP
ISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRADQHNQRAFNAQGFYITGDKVK
LSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVI
VLKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLA
LMS