Protein Info for CSW01_03945 in Vibrio cholerae E7946 ATCC 55056

Annotation: histidine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 TIGR00442: histidine--tRNA ligase" amino acids 6 to 410 (405 residues), 531.1 bits, see alignment E=9.2e-164 PF13393: tRNA-synt_His" amino acids 10 to 312 (303 residues), 147.8 bits, see alignment E=6.8e-47 PF00587: tRNA-synt_2b" amino acids 68 to 315 (248 residues), 100.5 bits, see alignment E=1.8e-32 PF03129: HGTP_anticodon" amino acids 330 to 418 (89 residues), 47.5 bits, see alignment E=2.4e-16

Best Hits

Swiss-Prot: 100% identical to SYH_VIBCH: Histidine--tRNA ligase (hisS) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 100% identity to vcm:VCM66_0718)

MetaCyc: 74% identical to histidine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Histidine--tRNA ligase. [EC: 6.1.1.21]

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (422 amino acids)

>CSW01_03945 histidine--tRNA ligase (Vibrio cholerae E7946 ATCC 55056)
MAKTIQAIRGMNDCLPTQSPLWQKVEGVVKNVISAYGYSEVRMPIVEMTHLFSRAIGEVT
DVVEKEMYTFEDRNGDSLTLRPEGTAGCVRSGIENGLLYNQEQRLWYMGPMFRHERPQKG
RYRQFHQCGVEVFGLDGPDVDAELIMMTARLWRELGIAQHVRLELNSIGSLEARANYRTA
LIDYLEQYQNVLDEDCKRRMYTNPLRVLDSKNPDVQAILGDAPQLSDYLDAESKQHFAGL
CELLDAAGIEYTVNQRLVRGLDYYNRTVFEWITESLGSQGTVCGGGRYDGLVEQLGGKPT
PAVGFAMGLERLVLMMETLGNTDVRRSVDVYMVTAGEGTMMAGMKLAEQLREQVPGLRVM
THFGGGNFKKQFKRADKVGAAIALVLGEDEVAAQTVVVKDLAGGEQNTVAQAEVAKLLAH
LA