Protein Info for CSW01_03780 in Vibrio cholerae E7946 ATCC 55056

Annotation: polyphosphate kinase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 701 TIGR03705: polyphosphate kinase 1" amino acids 7 to 677 (671 residues), 883.3 bits, see alignment E=5.8e-270 PF13089: PP_kinase_N" amino acids 7 to 109 (103 residues), 103.4 bits, see alignment E=2.1e-33 PF02503: PP_kinase" amino acids 119 to 303 (185 residues), 169.5 bits, see alignment E=1.6e-53 PF17941: PP_kinase_C_1" amino acids 330 to 492 (163 residues), 238.1 bits, see alignment E=1e-74 PF13091: PLDc_2" amino acids 366 to 487 (122 residues), 33.4 bits, see alignment E=9.6e-12 PF13090: PP_kinase_C" amino acids 501 to 672 (172 residues), 227.3 bits, see alignment E=2.1e-71

Best Hits

Swiss-Prot: 100% identical to PPK1_VIBCH: Polyphosphate kinase (ppk) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 100% identity to vch:VC0723)

MetaCyc: 64% identical to polyphosphate kinase (Escherichia coli K-12 substr. MG1655)
Polyphosphate kinase. [EC: 2.7.4.1]

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.1

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (701 amino acids)

>CSW01_03780 polyphosphate kinase 1 (Vibrio cholerae E7946 ATCC 55056)
MSADKLYIDKELSWLSFNERVLQEAADKTVPLIERIRFLGIFSNNLDEFYKVRFADVKRQ
ILINRERGGNDISKHLLSRMQSKALKLNQDFDNLYNELILEMARRRIFLVNETQLDEIQL
KWVKKYFHKEVLPHVTPIMLRDDIDVMQFLKDEYAYIAVEMRSGDEFKYALIEIPTDQLP
RFVMLPEQKGKRRKTIILLDNIIRLCLDEIFRGFYDYDTLNGYAMKMTRDAEYDLRHEVE
YSLLEQMSEGLSQRLTALPVRFVYEREMPEAMLKFLCYKLKISHYDSLIPGGRYHNFKDF
ISFPNVGRDYLENKPLPPMTCADFEGYANAFDAIRAQDILLHYPYHSFEHMTELVRQASF
DPKVVSIKINIYRVAKDSKLMNSLVDAVHNGKRVVVVVELQARFDEEANIEWSRILTDAG
VHVIFGVPGMKIHAKLLLITRKEGDEFVRYAHIGTGNFHERTARIYTDFALLTANQELAA
EVRAVFGYIENPFRPVKFNHLIVSPRNSRTQIYRLLDSEIANAKAGKKAAITLKVNNLVD
KGLINKLYGASAAGVKIRMIIRGMCSLVPGVEGVSDNIEIISIIDRFLEHPRVLVVHNDG
NPQVFISSADWMERNIDHRIEVMAPIRDERLKQRIIDILNIQFIDTVKARRIDKEMSNQY
VERGNRRKVRSQIAIYDYLKNVEKQTRKAKGQQETNDNSSQ