Protein Info for CSW01_03565 in Vibrio cholerae E7946 ATCC 55056

Annotation: lipid II flippase MurJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 525 transmembrane" amino acids 10 to 27 (18 residues), see Phobius details amino acids 33 to 51 (19 residues), see Phobius details amino acids 98 to 124 (27 residues), see Phobius details amino acids 143 to 165 (23 residues), see Phobius details amino acids 172 to 192 (21 residues), see Phobius details amino acids 204 to 225 (22 residues), see Phobius details amino acids 246 to 268 (23 residues), see Phobius details amino acids 288 to 306 (19 residues), see Phobius details amino acids 327 to 351 (25 residues), see Phobius details amino acids 365 to 385 (21 residues), see Phobius details amino acids 398 to 418 (21 residues), see Phobius details amino acids 424 to 443 (20 residues), see Phobius details amino acids 455 to 476 (22 residues), see Phobius details amino acids 495 to 515 (21 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 10 to 516 (507 residues), 453.6 bits, see alignment E=4.5e-140 PF03023: MurJ" amino acids 35 to 483 (449 residues), 468.4 bits, see alignment E=2.1e-144 PF13440: Polysacc_synt_3" amino acids 231 to 351 (121 residues), 30.1 bits, see alignment E=3e-11

Best Hits

Swiss-Prot: 100% identical to MURJ_VIBCH: Probable lipid II flippase MurJ (murJ) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K03980, virulence factor (inferred from 100% identity to vch:VC0680)

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (525 amino acids)

>CSW01_03565 lipid II flippase MurJ (Vibrio cholerae E7946 ATCC 55056)
MFEVTVSKRLLKSGIIVSAMTLISRVLGLVRDVVVANLMGAGASADVFFFANRIPNFLRR
LFAEGAFSQAFVPVLTEYHASGDINKTRDLIARASGTLGVLVTIVTLIGVLGSGAVTALF
GAGWFLDWLNGGPAAGKFELASLLLKITFPYLWFITFVALSGAILNTLGKFAVSSFTPVF
LNVMMILCAWYLSPNLEQPEVGLAIGVFLGGLVQFLFQLPFLIKAGVLVRPKWGWKDPGV
VKIRTLMIPALFGVSVSQINLLFDSFVASFLQTGSISWLYYSDRLLEFPLGLFGIAIATV
ILPALSRKHVDAHSDGFAHTMDWGIRMVTFLGIPAMLGLMVLAKPMLMVLFMRGEFTPSD
VEQASYSLLAYSSGLLSFMLIKVLAPGYYSRQDTKTPVRYGIIAMVSNIVLNAIFAWFYG
YVGLAVATSMSAFLNMALLYRGLHLQGVYHLTRKTVWFVARLAMAGAVMTGALLWQLDTM
ATWLSWGISQRALTLTGLIGLGVASYLAILLLLGVRLKDLKAATE