Protein Info for CSW01_03495 in Vibrio cholerae E7946 ATCC 55056

Annotation: lysine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 510 TIGR00499: lysine--tRNA ligase" amino acids 19 to 509 (491 residues), 664.6 bits, see alignment E=4.4e-204 PF01336: tRNA_anti-codon" amino acids 73 to 145 (73 residues), 38.8 bits, see alignment E=7.5e-14 PF00152: tRNA-synt_2" amino acids 161 to 506 (346 residues), 312 bits, see alignment E=3.9e-97

Best Hits

Swiss-Prot: 100% identical to SYK_VIBCH: Lysine--tRNA ligase (lysS) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 100% identity to vcj:VCD_003655)

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.6

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (510 amino acids)

>CSW01_03495 lysine--tRNA ligase (Vibrio cholerae E7946 ATCC 55056)
MTDAVQNEINQEQIAQEENKLIAERRSKLDHIRKNCKANGHPNSFRRDSLAGDLQKKFGE
KSKEELEALNHVVSIAGRVMAKRGPFLVIQETSGRIQAYASKEVQQELKDKYQGLDIGDI
IGVQGALHKSGKGDLYVNMEQFQLLTKALRPLPEKFHGLTDQEMRYRQRYVDLIVNENSR
NAFIVRSKVMSAIRNFMISKQFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPE
LYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSV
ALEVLGSTSMPYGEHTVEFGGKYARMSMFDAIKHYNPNHAQIQALTEEDIQNRDLMVSIA
KSVHVDVEPFWTCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRSDDNPFFTDRFE
FFIGGREVANGFSELNDAEDQDARFKAQVEAKESGDDEAMFYDADYITALEHGLPPTAGQ
GIGIDRLVMLLTNTHTIRDVILFPAMRPQQ