Protein Info for CSW01_02965 in Vibrio cholerae E7946 ATCC 55056

Annotation: insulinase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 952 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00675: Peptidase_M16" amino acids 64 to 181 (118 residues), 64.3 bits, see alignment E=1.2e-21 amino acids 540 to 648 (109 residues), 39.2 bits, see alignment E=6.8e-14 PF05193: Peptidase_M16_C" amino acids 220 to 395 (176 residues), 131.1 bits, see alignment E=4.5e-42 amino acids 687 to 858 (172 residues), 103.4 bits, see alignment E=1.4e-33

Best Hits

KEGG orthology group: K07263, zinc protease [EC: 3.4.24.-] (inferred from 100% identity to vcj:VCD_001051)

Predicted SEED Role

"Protease, insulinase family/protease, insulinase family"

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (952 amino acids)

>CSW01_02965 insulinase family protein (Vibrio cholerae E7946 ATCC 55056)
MMRKVALFGFSLLLLAGCSSSDSSLPLFSSLPKGVTLVEEVKAEPGKVMIPYSKYRLDNG
LTVILSPDDSDPLVHVDVTYHVGSAREEIGKSGFAHFFEHMMFQGSKHVGDQQHFRLITE
AGGSLNGTTNRDRTNYFETVPANQLEKMLWLEADRMGFLLDAVSQRKFEIQRDTVKNERA
QNYDNRPYGLMWEKMGEALYPEGHPYSWQTIGYVSDLDRVDVNDLKAFFLRWYGPNNAVL
TIGGDLDVKQTLAWVQKYFGSIPKGPDVVDAPKQPARLSEDRFITLEDRVQQPMLLIGWP
TQYWGAEDQVALDALASALGSGNNSLLYQELVKTQKAVDAGAFQDCAELACTFYVYAMAP
SGAKGKLAPLYQETLQVLEKFKQQGVSASRLEQIIGSEEASAVFALESVKGKVSQLAANQ
TFFDQPDRIESQLEKIRAVTPESVKQVFTRYLDGQPKVTLSVVAKGKTDFAVRPATFITP
ERQLPEYQKIGDEQLAYREVKDSFDRSQMPQVAQAVQPRLPKLYDVYFDNGVQLLGTQTR
ETPTVLIEIQLPAGERQVAMGKEGLANLTASLLQEGSQNRSAEAIQAQLDKLGSSIQVVA
GAYSTSIVVSSLKKNLPETLQISQEMLLKPAFKQSDFARLQQQMLQGVVYQHQQPSWLAS
QATRQVLWGESLFARSGDGTQASISALTLKDVKQFYRQHYTPHGAQIAVVGDISAREIRQ
QLQFIADWKGEAAPLINPQVVPTLTKQKIYLVDKPGAPQSIIRMVRKGLPFDATGELYLT
QLANFNLAGNFNSRINQNLREDKGYTYGAGSYFASNREIGAIVFNAPVRADVTVEAIQEM
IKEMHHFSQAGMSEEEMKFLRLAVGQQDALMYETPAQKAQLVSSILTYSLDRDYLQQRNE
IVKSVDRSTLNELAAKWFNPEDYQIIVVGDAKRLKPQLEKLGIPLEELEIIR