Protein Info for CSW01_02850 in Vibrio cholerae E7946 ATCC 55056

Annotation: sulfate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 21 to 330 (310 residues), 445.7 bits, see alignment E=4.7e-138 PF13531: SBP_bac_11" amino acids 45 to 281 (237 residues), 117 bits, see alignment E=5.8e-38

Best Hits

Swiss-Prot: 62% identical to CYSP_SALTY: Thiosulfate-binding protein (cysP) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 100% identity to vcm:VCM66_0496)

MetaCyc: 61% identical to thiosulfate/sulfate ABC transporter periplasmic binding protein CysP (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (333 amino acids)

>CSW01_02850 sulfate ABC transporter substrate-binding protein (Vibrio cholerae E7946 ATCC 55056)
MNRIKSTLTALLLAGSFQASAADQTILNSSYDIARELFVAYNPVFAEHWQQKTGKTVEIK
QSHAGSSAQARSILQGLAADVVTFNQVTDVQILHDKGKLIPANWQTLLPNASSPYYSTTA
FLVRKGNPKNIQDWSDLVRDDVKSVFPNPKTSGNARYTYLAALGFAQKQFGQDNQVQQDE
FLKKFLANVAVFDTGGRGATTSFVERGIGDVLITFESEVNNIRQQYGADDYQVVVPKTSI
LAEFPVAVVEKVAKRNGTYDVATEYVSYLYSEPAQRLLAEFNYRVHDAKVQAEFAERFPA
VELLTVEGIAGGWEQAMQTQFANGAKLDQLLRR