Protein Info for CSW01_02785 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional dihydroneopterin aldolase/7,8-dihydroneopterin epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00526: FolB domain
  • amino acids 13 to 128 (116 residues), 123.6 bits, E=5.2e-40
• TIGR00525: dihydroneopterin aldolase
  • amino acids 14 to 126 (113 residues), 100.7 bits, E=7.3e-33
• PF02152: FolB
  • amino acids 16 to 125 (110 residues), 115.4 bits, E=1e-37

Best Hits

Swiss-Prot: 70% identical to FOLB_ECOL6: Dihydroneopterin aldolase (folB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01633, dihydroneopterin aldolase [EC: 4.1.2.25] (inferred from 100% identity to vcm:VCM66_0482)

MetaCyc: 70% identical to dihydroneopterin aldolase (Escherichia coli K-12 substr. MG1655)
RXN-10856 [EC: 5.1.99.8]; Dihydroneopterin aldolase. [EC: 5.1.99.8, 4.1.2.25]

Predicted SEED Role

"Dihydroneopterin aldolase (EC 4.1.2.25)" in subsystem Folate Biosynthesis (EC 4.1.2.25)

MetaCyc Pathways

• superpathway of chorismate metabolism (51/59 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I (3/5 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis II (Methanocaldococcus) (2/7 steps found)
• tetrahydromethanopterin biosynthesis (3/14 steps found)

KEGG Metabolic Maps

• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.2.25 or 5.1.99.8

Protein Sequence (129 amino acids)

>CSW01_02785 bifunctional dihydroneopterin aldolase/7,8-dihydroneopterin epimerase (Vibrio cholerae E7946 ATCC 55056)
MGIRPVNRKRLSMDKVFIEQLEVITTIGVYDWEQQIKQKLVLDLEMAHDNRAAGKSDDVA
DALDYAQVSQAVLEHIEQGRFLLVERVAEEVAELIMTRFAVPWLRIRLTKPGAVPQAKGV
GVIIERARG