Protein Info for CSW01_02765 in Vibrio cholerae E7946 ATCC 55056

Annotation: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 339 TIGR03723: tRNA threonylcarbamoyl adenosine modification protein TsaD" amino acids 3 to 313 (311 residues), 451.3 bits, see alignment E=1.7e-139 TIGR00329: metallohydrolase, glycoprotease/Kae1 family" amino acids 5 to 306 (302 residues), 417.4 bits, see alignment E=3.5e-129 PF00814: TsaD" amino acids 24 to 307 (284 residues), 342.7 bits, see alignment E=1.8e-106 PF22521: HypF_C_2" amino acids 220 to 302 (83 residues), 39.5 bits, see alignment E=5.9e-14

Best Hits

Swiss-Prot: 100% identical to TSAD_VIBC3: tRNA N6-adenosine threonylcarbamoyltransferase (tsaD) from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)

KEGG orthology group: K01409, O-sialoglycoprotein endopeptidase [EC: 3.4.24.57] (inferred from 100% identity to vcj:VCD_001084)

MetaCyc: 76% identical to N6-L-threonylcarbamoyladenine synthase, TsaD subunit (Escherichia coli K-12 substr. MG1655)
RXN-14570 [EC: 2.3.1.234]

Predicted SEED Role

"TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.234 or 3.4.24.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (339 amino acids)

>CSW01_02765 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD (Vibrio cholerae E7946 ATCC 55056)
MRIIGIETSCDETGIAIYDDEKGLLSHKLYSQVKLHADYGGVVPELASRDHVKKTIPLIK
AAMAEANVTPQDLDGVAFTAGPGLVGALLVGATIGRSLAYAWDVPAVPVHHMEGHLLAPM
LEENPPPFPFVALLVSGGHTMLVEVKNIGEYRILGESIDDAAGEAFDKTAKLMGLDYPGG
PLLAKLAEKGTPGRFKFPRPMTDRPGLDMSFSGLKTFTANTIAANGDDEQTRADIAYAFQ
EAVCDTLVIKCKRALEETGLKRVVIAGGVSANKQLRADLEKLAKKIGGEVYYPRTEFCTD
NGAMIAYAGMQRLKNGDVCELGLQARPRWPIDQLTSIQK