Protein Info for CSW01_02015 in Vibrio cholerae E7946 ATCC 55056

Annotation: assimilatory sulfite reductase (NADPH) hemoprotein subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02041: sulfite reductase (NADPH) hemoprotein, beta-component
  • amino acids 24 to 565 (542 residues), 925.8 bits, E=3.6e-283
• PF03460: NIR_SIR_ferr
  • amino acids 84 to 143 (60 residues), 49.8 bits, 2.4e-17
  • amino acids 358 to 422 (65 residues), 40.9 bits, E=1.4e-14
• PF01077: NIR_SIR
  • amino acids 178 to 334 (157 residues), 140 bits, E=4.6e-45

Best Hits

Swiss-Prot: 100% identical to CYSI_VIBCH: Sulfite reductase [NADPH] hemoprotein beta-component (cysI) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 100% identity to vcj:VCD_001238)

MetaCyc: 72% identical to sulfite reductase, hemoprotein subunit (Escherichia coli K-12 substr. MG1655)
Sulfite reductase (NADPH). [EC: 1.8.1.2]

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (5/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Protein Sequence (577 amino acids)

>CSW01_02015 assimilatory sulfite reductase (NADPH) hemoprotein subunit (Vibrio cholerae E7946 ATCC 55056)
MSANQNPSVQEVLGEVLGPWSDNERLKRESHFLRGTIEQDLQDRITGGFTADNFQLIRFH
GMYQQDDRDIRAERSKQKLEPLHNVMLRARMPGGIITPHQWLAIDKFATEHTLYGSIRLT
TRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLCTSNPVESELHLQAYEWAK
KISEHLLPKTRAYAEIWLDGEKIEGPDEEPILGSNYLPRKFKTTVVIPPHNDVDVHANDL
NFVAIGENGQLVGFNVLVGGGLAMTHGDTSTYPRRADDFGFIPLEKTLEVAAAVVSTQRD
WGNRSNRKNAKTKYTLDRVGVEVFKAEVEKRAGITFAPSRAYEFTSRGDRIGWVEGIDGK
HHLTLFIENGRILDFPGKPLKTGVAEIAKVHQGDFRMTANQNLIVAGVPADQKPHIEQLA
REHGLIDDGVSEQRINSMACVAFPTCPLAMAEAERFLPSFVTEVEGILAKHALPKEENII
LRVTGCPNGCGRAMLAEIGLVGKAPGRYNLHLGGNRNGTRIPKMYKENITDTQILQEIDE
LVGRWASERLDGEGFGDFTIRAGIIEEVIISKRDFYA