Protein Info for CSW01_00840 in Vibrio cholerae E7946 ATCC 55056

Annotation: ketol-acid reductoisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07991: IlvN
  • amino acids 35 to 204 (170 residues), 193 bits, E=2.9e-61
• TIGR00465: ketol-acid reductoisomerase
  • amino acids 35 to 367 (333 residues), 410.3 bits, E=2.3e-127
• PF01450: IlvC
  • amino acids 210 to 341 (132 residues), 82.2 bits, E=4.8e-27
  • amino acids 360 to 485 (126 residues), 46.8 bits, E=4e-16

Best Hits

Swiss-Prot: 100% identical to ILVC_VIBCM: Ketol-acid reductoisomerase (NADP(+)) (ilvC) from Vibrio cholerae serotype O1 (strain M66-2)

KEGG orthology group: K00053, ketol-acid reductoisomerase [EC: 1.1.1.86] (inferred from 100% identity to vch:VC0162)

MetaCyc: 80% identical to ketol-acid reductoisomerase (NADP+) (Escherichia coli K-12 substr. MG1655)
Ketol-acid reductoisomerase. [EC: 1.1.1.86]; 1.1.1.86 [EC: 1.1.1.86]; 2-dehydropantoate 2-reductase. [EC: 1.1.1.86, 1.1.1.169]

Predicted SEED Role

"Ketol-acid reductoisomerase (EC 1.1.1.86)" in subsystem Branched-Chain Amino Acid Biosynthesis or Coenzyme A Biosynthesis (EC 1.1.1.86)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• superpathway of coenzyme A biosynthesis I (bacteria) (8/9 steps found)
• L-valine biosynthesis (4/4 steps found)
• phosphopantothenate biosynthesis I (4/4 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• phosphopantothenate biosynthesis III (archaea) (2/4 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (5/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.169

Use Curated BLAST to search for 1.1.1.169 or 1.1.1.86

Protein Sequence (494 amino acids)

>CSW01_00840 ketol-acid reductoisomerase (Vibrio cholerae E7946 ATCC 55056)
MANYFNTLNLREQLDQLGRCRFMAREEFATEADYLKGKKVVIVGCGAQGLNQGLNMRDSG
LDVSYALRQAAIDEQRQSFKNAKNNGFNVGSYEQLIPTADLVINLTPDKQHTSVVNAVMP
LMKQGAALGYSHGFNIVEEGMQIRKDITVVMVAPKCPGTEVREEYKRGFGVPTLIAVHPE
NDPQGEGWEIAKAWAAATGGHRAGCLASSFVAEVKSDLMGEQTILCGMLQAGSIVCYEKM
VADGIDPGYAGKLLQFGWETITEALKFGGITHMMDRLSNPAKIKAFELSEELKDLMRPLY
NKHMDDIISGHFSSTMMADWANDDKDLFGWRAETAETAFENYPTTDVKIAEQEYFDNGIL
MIAMVRAGVELAFEAMTASGIIDESAYYESLHELPLIANTVARKRLYEMNVVISDTAEYG
NYLFANVAVPLLREKFMPKVGTDVIGKGLGVVSNQVDNATLIEVNSIIRNHPVEYIGEEL
RGYMKDMKRIAVGD