Protein Info for CSW01_00740 in Vibrio cholerae E7946 ATCC 55056

Annotation: 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 199 TIGR00095: 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD" amino acids 15 to 196 (182 residues), 193.2 bits, see alignment E=1.8e-61 PF03602: Cons_hypoth95" amino acids 19 to 194 (176 residues), 219.2 bits, see alignment E=1.2e-68 PF05175: MTS" amino acids 46 to 146 (101 residues), 44.7 bits, see alignment E=4.1e-15 PF02475: Met_10" amino acids 59 to 122 (64 residues), 24.1 bits, see alignment E=9.9e-09 PF13847: Methyltransf_31" amino acids 60 to 156 (97 residues), 32.9 bits, see alignment E=1.9e-11 PF13649: Methyltransf_25" amino acids 64 to 153 (90 residues), 28.1 bits, see alignment E=9.8e-10

Best Hits

Swiss-Prot: 56% identical to RSMD_ECOLI: Ribosomal RNA small subunit methyltransferase D (rsmD) from Escherichia coli (strain K12)

KEGG orthology group: K08316, ribosomal RNA small subunit methyltransferase D [EC: 2.1.1.171] (inferred from 100% identity to vco:VC0395_A2375)

MetaCyc: 56% identical to 16S rRNA m2G966 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6515 [EC: 2.1.1.171]

Predicted SEED Role

"16S rRNA (guanine(966)-N(2))-methyltransferase (EC 2.1.1.171)" (EC 2.1.1.171)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.171

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (199 amino acids)

>CSW01_00740 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (Vibrio cholerae E7946 ATCC 55056)
MQRRRQPNPSQKKAPTGQVRIISGLWRGRKLPVHDAEGLRPTTDRVKETLFNWLAQEVPN
ARCLDLFAGSGGLGFECASRQANQVTMLELNPLAFQQLKTNVQSLKAENIELIHTDALAF
LRQSGKPYDVVFIDPPFRQGLLQETTQLLEQNGWLAPQAMIYIECEKELPLTELPPHWHL
HREKSAGQVCYRLFIREEQ