Protein Info for CSW01_00530 in Vibrio cholerae E7946 ATCC 55056

Annotation: rhomboid family intramembrane serine protease GlpG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 277 transmembrane" amino acids 97 to 118 (22 residues), see Phobius details amino acids 138 to 162 (25 residues), see Phobius details amino acids 174 to 194 (21 residues), see Phobius details amino acids 200 to 217 (18 residues), see Phobius details amino acids 224 to 244 (21 residues), see Phobius details amino acids 249 to 269 (21 residues), see Phobius details PF12122: Rhomboid_N" amino acids 1 to 86 (86 residues), 119.5 bits, see alignment E=6.5e-39 TIGR04239: rhomboid family protease GlpG" amino acids 4 to 272 (269 residues), 378.4 bits, see alignment E=1.2e-117 PF01694: Rhomboid" amino acids 134 to 273 (140 residues), 124.4 bits, see alignment E=4.1e-40

Best Hits

Swiss-Prot: 46% identical to GLPG_YERPB: Rhomboid protease GlpG (glpG) from Yersinia pseudotuberculosis serotype IB (strain PB1/+)

KEGG orthology group: K02441, GlpG protein (inferred from 100% identity to vcm:VCM66_0099)

MetaCyc: 44% identical to rhomboid protease GlpG (Escherichia coli K-12 substr. MG1655)
Rhomboid protease. [EC: 3.4.21.105]

Predicted SEED Role

"GlpG protein (membrane protein of glp regulon)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.21.105

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (277 amino acids)

>CSW01_00530 rhomboid family intramembrane serine protease GlpG (Vibrio cholerae E7946 ATCC 55056)
MHLLTTFNNPRAAQAFIDYMAAHHIEIQMMPDAGGQFTLWVIQDQHIETAQAELALFLEN
PYAEKYQAASWEVADQKRPQFHYASPNLLSLIKAKAGVFTLFIMALCIIIFTLQTFGAGD
EVFNALHFPALAGQQWQIWRWVSHALLHFSVMHIAFNLLWWWQFGGDLEQRLGSVRLIKL
FVVSAIISGAGQYWVEGANFGGLSGVVYALAGYLWILGQRAPQLGLSIPRSLMGFMLIWL
VLGYVQPFMAIANTAHLAGLISGVVLAWFDSQRDQQA