Protein Info for CSW01_00500 in Vibrio cholerae E7946 ATCC 55056

Annotation: glycerol-3-phosphate 1-O-acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03703: glycerol-3-phosphate O-acyltransferase
  • amino acids 11 to 799 (789 residues), 1208.5 bits, E=0
• PF01553: Acyltransferase
  • amino acids 294 to 424 (131 residues), 91.7 bits, E=3.4e-30
• PF19277: GPAT_C
  • amino acids 435 to 771 (337 residues), 376.1 bits, E=1.8e-116

Best Hits

Swiss-Prot: 100% identical to PLSB_VIBCM: Glycerol-3-phosphate acyltransferase (plsB) from Vibrio cholerae serotype O1 (strain M66-2)

KEGG orthology group: K00631, glycerol-3-phosphate O-acyltransferase [EC: 2.3.1.15] (inferred from 100% identity to vch:VC0093)

MetaCyc: 58% identical to glycerol-3-phosphate 1-O-acyltransferase (Escherichia coli K-12 substr. MG1655)
Glycerol-3-phosphate O-acyltransferase. [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]

Predicted SEED Role

"Glycerol-3-phosphate acyltransferase (EC 2.3.1.15)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.3.1.15)

MetaCyc Pathways

• phosphatidylglycerol biosynthesis I (6/6 steps found)
• phosphatidylglycerol biosynthesis II (6/6 steps found)
• superpathway of phospholipid biosynthesis III (E. coli) (10/12 steps found)
• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• superpathway of cardiolipin biosynthesis (bacteria) (10/13 steps found)
• oleate biosynthesis III (cyanobacteria) (2/3 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
• palmitoyl ethanolamide biosynthesis (2/6 steps found)
• superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
• stigma estolide biosynthesis (2/7 steps found)
• anandamide biosynthesis II (2/8 steps found)
• anandamide biosynthesis I (3/12 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15

Protein Sequence (808 amino acids)

>CSW01_00500 glycerol-3-phosphate 1-O-acyltransferase (Vibrio cholerae E7946 ATCC 55056)
MSSGHLLSRSLLKLPMSVLVKGTAIPSNPIQDLDIDTHKPVIYALPFRSNVDLLTLQTHA
KEAGLPDPLEPLMLNGKAFQRYVFIASRPTLLSSDQHVPSDSIALFSELLTEHKLDSELD
VQVIPATVLWGRKPGKEGQERPYLQALNGPEKALAVLASGRDCLVRFSPVVSMRYMADTH
GTDASIAHKLARVARIHFSRQKLAASGPNLPQRAQLFARLMNSPAIEKAIADEAKSKQIP
LEKARKEAHDILDEIAADFSYSLVKKGDRILGWLWNRIYQGLNINNAATVRRLAQDGHEI
VYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGAP
LYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTGMLAMTIQAMLRGLNRPVTLV
PVYIGYEHVMEVGTYAKELRGKRKEKENAGLVLRTLRKLRNFGQGYVNFGEPIPLNQFLN
ETVPQWTQDIDPMGESKPQWMTPTVNKLANRMMTHINDAAAVNAMTLCATALLASRQRAL
ARDNLIKQVDCYLSLLRNVPYSATSTLPSESAEKLVEHAESLDKFVVETDTMGDIISLDR
NQSILMTYYRNNIIHLLALPSLIAQLLIRQQSVSLEKVQATVAQIYPFLKQELFLRFEAE
ELNDLVLRYVAELARQGLVTVEGKTVTLNQAQTQVLMLLGRIISETLQRYAIALNLLVSC
PHLGKAELEEKSQEVAQRLGRLHGINAPEFFDKGVFASLFVTLQEQGYLDDQGRCVLETA
KPLSRQLYALIYPEVRMTIQESLCQVDA