Protein Info for CCNA_03865 in Caulobacter crescentus NA1000

Annotation: leucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 861 TIGR00396: leucine--tRNA ligase" amino acids 4 to 860 (857 residues), 1001.7 bits, see alignment E=1.5e-305 PF00133: tRNA-synt_1" amino acids 13 to 224 (212 residues), 102.6 bits, see alignment E=4.3e-33 amino acids 423 to 582 (160 residues), 29.7 bits, see alignment E=6e-11 amino acids 622 to 662 (41 residues), 31.3 bits, see alignment 2e-11 PF09334: tRNA-synt_1g" amino acids 37 to 171 (135 residues), 69.8 bits, see alignment E=3.8e-23 amino acids 622 to 665 (44 residues), 22.7 bits, see alignment 7.7e-09 PF13603: tRNA-synt_1_2" amino acids 221 to 410 (190 residues), 215.6 bits, see alignment E=8.6e-68 PF08264: Anticodon_1" amino acids 703 to 824 (122 residues), 71.9 bits, see alignment E=1.1e-23

Best Hits

Swiss-Prot: 100% identical to SYL_CAUVN: Leucine--tRNA ligase (leuS) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 100% identity to ccr:CC_3749)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8GW27 at UniProt or InterPro

Protein Sequence (861 amino acids)

>CCNA_03865 leucyl-tRNA synthetase (Caulobacter crescentus NA1000)
MARYNPKDTEPKWREAWAKADVFKTGEINDGRPKYYVLEMFPYPSGRIHMGHVRNYAMGD
VVARYKRAQGFNVLHPMGWDAFGMPAENAAMERGVHPKGWTYDNIAAMREQLKSLGISVD
WSREFATCDPEYYGKQQAWFLRLLKRGLVYRKEASVNWDPVDMTVLANEQVIDGKGWRSG
AVVEKRKLTQWFLRITDYADALIDGLKTLDRWPDKVRLMQENWIGRSKGLRFKFQFDGEA
PDGMAEGLEVYTTRPDTLFGASFVGIAPEHPLAEQLAAANPQIQTFIADCRKGGTSEAEI
ESAEKLGYDTGLRVKHPLDPSITLPVWIANFILMDYGTGAIFACPAHDQRDLDFARKYDL
PVLPVVLPNGEDPATFTVGKEAYVGPGKIFNSKFLDGLDVEAAKAEAIARIEAANQGQGA
TVYRLRDWGVSRQRYWGCPIPVIHCEACGVVPVPEDQLPVALPDDVTFDKPGNPLLRHPT
WRHTTCPSCGGKAERETDTLDTFIDSSWYFARFADTQAAEPVGKDAADHWLPVDQYIGGV
EHAILHLLYARFITRALKDEGLLSVEEPFAGLFTQGMVTHEAYKNEAGEWVEPSDVVITT
EGSTRTAKHAKTGAPIIIGDIEKMSKSKKNVVAPEDIFEAYGVDSARLFVMSDSPPERDV
QWTNSGVEGSWRFTHRLWNEFDSQPAGDFAHDDSDEAALALRKAAHKLIGFVTDSIEGFR
FNSGVARLYEFLNALKAAPAEGASQAVLAARAEALNILARLVAPFTPHLAEEAWARMGGE
GMVVDAPWPKADPALAADDERVLPIQINGKRRGEIKVKAGAPDDEVTKIALADPNVMAHL
EGLTVRKVIVVKDRIVNIVAN