Protein Info for CCNA_03744 in Caulobacter crescentus NA1000

Annotation: dTDP-glucose 4,6-dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 7 to 176 (170 residues), 47 bits, E=6.8e-16
• PF01370: Epimerase
  • amino acids 8 to 255 (248 residues), 223.7 bits, E=8.5e-70
• TIGR01181: dTDP-glucose 4,6-dehydratase
  • amino acids 8 to 341 (334 residues), 469.9 bits, E=1.9e-145
• PF02719: Polysacc_synt_2
  • amino acids 8 to 119 (112 residues), 56.6 bits, E=8.4e-19
• PF01073: 3Beta_HSD
  • amino acids 9 to 246 (238 residues), 41.9 bits, E=2.3e-14
• PF16363: GDP_Man_Dehyd
  • amino acids 9 to 327 (319 residues), 287.7 bits, E=5.3e-89
• PF07993: NAD_binding_4
  • amino acids 11 to 191 (181 residues), 26 bits, E=1.7e-09

Best Hits

Swiss-Prot: 63% identical to RFBB_SINFN: Probable dTDP-glucose 4,6-dehydratase (NGR_a03580) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K01710, dTDP-glucose 4,6-dehydratase [EC: 4.2.1.46] (inferred from 100% identity to ccr:CC_3629)

MetaCyc: 60% identical to dTDP-glucose 4,6-dehydratase 2 (Escherichia coli K-12 substr. MG1655)
dTDP-glucose 4,6-dehydratase. [EC: 4.2.1.46]

Predicted SEED Role

"dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 4.2.1.46)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• dTDP-4-O-demethyl-β-L-noviose biosynthesis (3/5 steps found)
• dTDP-β-D-fucofuranose biosynthesis (2/4 steps found)
• dTDP-6-deoxy-α-D-allose biosynthesis (2/4 steps found)
• dTDP-N-acetylthomosamine biosynthesis (2/4 steps found)
• dTDP-N-acetylviosamine biosynthesis (2/4 steps found)
• dTDP-L-daunosamine biosynthesis (3/6 steps found)
• dTDP-sibirosamine biosynthesis (3/6 steps found)
• dTDP-α-D-mycaminose biosynthesis (2/5 steps found)
• dTDP-3-acetamido-α-D-fucose biosynthesis (2/5 steps found)
• dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis (2/5 steps found)
• dTDP-β-L-digitoxose biosynthesis (3/7 steps found)
• dTDP-β-L-olivose biosynthesis (3/7 steps found)
• dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis (2/6 steps found)
• dTDP-D-desosamine biosynthesis (2/6 steps found)
• dTDP-β-L-4-epi-vancosamine biosynthesis (3/8 steps found)
• dTDP-β-L-megosamine biosynthesis (3/8 steps found)
• dTDP-β-L-mycarose biosynthesis (2/7 steps found)
• dTDP-α-D-forosamine biosynthesis (3/9 steps found)
• dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis (3/9 steps found)
• superpathway of enterobacterial common antigen biosynthesis (2/10 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (5/19 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)
• superpathway of erythromycin biosynthesis (2/19 steps found)
• superpathway of megalomicin A biosynthesis (3/22 steps found)
• superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis (3/23 steps found)

KEGG Metabolic Maps

• Biosynthesis of vancomycin group antibiotics
• Polyketide sugar unit biosynthesis
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.46

Use Curated BLAST to search for 4.2.1.46

Sequence Analysis Tools

See A0A0H3CDX1 at UniProt or InterPro

Protein Sequence (356 amino acids)

>CCNA_03744 dTDP-glucose 4,6-dehydratase (Caulobacter crescentus NA1000)
MSQQNLRVMVTGGSGFIGSAVCRHLAGQNNVAILNYDKLTYAASQASLAMLEGKADYQFV
QGDVADAARVCATIKAFRPDVVMHLAAESHVDRSITGPGDFIQTNIVGTYVMLQAALEHW
RGLEGEAKGRFRFHHISTDEVFGSLGAEGLFSETTPYDPRSPYSASKASSDHLARAWQHT
YGLPVVVSNCSNNYGPYHFPEKLIPLVTLNALEGKPLPVYGKGDNVRDWLHVEDHARALH
LIATKGVPGESYNVGGRNERTNLQVVEAICDILDELRPIPGQSRRDLITFVADRPGHDAR
YAIDATKLETELGWTAQETFDTGLRKTIQWYLDNEAWWAPLRERYAGQRLGLSKQA