Protein Info for CCNA_03734 in Caulobacter crescentus NA1000

Annotation: glutamine-dependent NAD synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 678 PF00795: CN_hydrolase" amino acids 17 to 285 (269 residues), 78.4 bits, see alignment E=6e-26 PF02540: NAD_synthase" amino acids 350 to 522 (173 residues), 78.4 bits, see alignment E=4.8e-26 TIGR00552: NAD+ synthetase" amino acids 355 to 521 (167 residues), 65.1 bits, see alignment E=2.9e-22

Best Hits

KEGG orthology group: K01950, NAD+ synthase (glutamine-hydrolysing) [EC: 6.3.5.1] (inferred from 100% identity to ccr:CC_3619)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5 or 6.3.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3CE54 at UniProt or InterPro

Protein Sequence (678 amino acids)

>CCNA_03734 glutamine-dependent NAD synthetase (Caulobacter crescentus NA1000)
MGSPSFFSPYRHGFVRVATAVPKVKLADPAANAQNVVALAREAHAEGVAVVVFPELGLTG
YTIDDLLQQEALLDAVEAAIATLTEASAGLAPMIVVGGPLRDAGRLYNTAIVIQGGKVLG
VVPKSFLPNYREFYERRWFTPGAGLTGKTLTLAGQTVPFGTDILFRGEGVAPFTVGVEIC
EDVWTPTPPSTAQALAGAEILLNLSASNITIGKSETRRLLCASQSSRMIAAYVYSAAGAG
ESSTDLAWDGHVDIHEMGALLAETPRFSTGPAWTFADVDVQRLRQERMRVGSFGDAMALS
PASTPFRIVPFAFDAPEGDLALARPIERFPFTPSDPARLRENCYEAYNIQVQGLARRLEA
SGLKKLVIGISGGLDSTQALLVAAKAMDQLGLPRSNILAYTLPGFATSDRTKSNAWALMK
AMAVTAAELDIRPAATQMLKDLDHPFGRGEAVYDVTFENVQAGLRTDYLFRLANHNAALV
VGTGDLSELALGWCTYGVGDHMSHYNPNCGAPKTLIQHLIRFVAHSGDVGAETTALLDDI
LATEISPELVPGEAVQATESFVGPYALQDFNLYYMTRYGMAPSKIAFLAWSAWHDADQGG
WPVGLPDNARRAYDLPEIKRWLELFLKRFFANQFKRSAVPNGPKISSGGALSPRGDWRMP
SDATADAWLAELRTNAPI