Protein Info for CCNA_03725 in Caulobacter crescentus NA1000

Annotation: ATP-dependent DNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 541 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF04675: DNA_ligase_A_N" amino acids 4 to 161 (158 residues), 26.8 bits, see alignment E=8.3e-10 TIGR04120: DNA ligase, ATP-dependent, PP_1105 family" amino acids 4 to 540 (537 residues), 845.3 bits, see alignment E=1.2e-258 PF01068: DNA_ligase_A_M" amino acids 210 to 406 (197 residues), 88.6 bits, see alignment E=7.1e-29 PF04679: DNA_ligase_A_C" amino acids 427 to 525 (99 residues), 36.7 bits, see alignment E=6.9e-13

Best Hits

KEGG orthology group: K01971, DNA ligase (ATP) [EC: 6.5.1.1] (inferred from 100% identity to ccr:CC_3610)

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1) LigC" in subsystem DNA Repair Base Excision (EC 6.5.1.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3CCB2 at UniProt or InterPro

Protein Sequence (541 amino acids)

>CCNA_03725 ATP-dependent DNA ligase (Caulobacter crescentus NA1000)
MSMRAFAHLLDRLSLTSSRNAKLILIQDHLRQTPDPDRGYALAALTGALSFNAAKPAFIR
KAIEARMDPQLFAWSYDFVGDLAETVSLVWPAPSSRKWLGANRPPELSEVIDALHSASRP
EVQRLIEGWLDALDPDGRWALLKLMTGGLRVGVSSRLAKQACANLGGVDIGEIEEIWHAL
APPYGDLFAWLEGRSERPSPDAPGRFRPVMLAQPLDEEVDFQKLDPADYLAEWKWDGIRV
QAVSERGERRLYTRTGDDISASFPDVVEALDFEGAIDGELLVLRDGALASFGDLQQRLNR
KTVDAKLLAAFPAGIRAYDLLMDGETDLRALPFETRRARLEAFVASLKTSRIDLSPLQPF
STWKALAALRLEPPEGDARLSEGLMLKRRDSVYEPGRPKGPWFKWKRDPRLIDAVLMYAQ
RGHGKRSSFYSDYTFGVWREDEDGRRTLTPVGKAYFGFTDEELKQIDKFVRDHTIERFGP
VRSVRADADVGLIFEVAFEGLQRSSRHKSGVAMRFPRINRIRWDKPAREADELATLEAML
D