Protein Info for CCNA_03655 in Caulobacter crescentus NA1000

Annotation: folylpolyglutamate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 27 to 430 (404 residues), 410.6 bits, E=4.8e-127
• PF08245: Mur_ligase_M
  • amino acids 50 to 265 (216 residues), 29.3 bits, E=4.1e-11

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 100% identity to ccr:CC_3541)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of chorismate metabolism (38/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Sequence Analysis Tools

See A0A0H3CDY1 at UniProt or InterPro

Protein Sequence (437 amino acids)

>CCNA_03655 folylpolyglutamate synthase/dihydrofolate synthase (Caulobacter crescentus NA1000)
MTDHLRAHDAALARLQALHPKLIDLSLDRMRRLCAALGDPQDRLPPVIHVAGTNGKGSTV
AFLRAMAEAAGLKVHVFTSPHLVRFSERIRLAGTLITDAHLADVLDRVEQANAGEPITFF
EITTAAAFVAFAEVPADLCIIEVGLGGLLDATNVVTPKVSVIAPVDLDHREFLGDTIGQI
AGEKAGIIKSGAPAVSARQHEDAEPVIEAAADRAGVSLTLMGRDFDAWSERGRLLVQMQD
RLLDLPAPSLPGEHQFANAGLAVAALLALGDPRIDEAAMGRGLGSTVWPARFQRLTAGPL
AERAKAAGADLWLDGGHNPHAGLAVSRAVADLAARDGRPVALISGLLANKDAAGFFGAFR
GVATKVFTVTFEGHAAASAADTAAAAELAGLRASACDSVAEALDRALQLEPAPHILICGS
LYLAGDVLAMSPETWPV