Protein Info for CCNA_03628 in Caulobacter crescentus NA1000

Annotation: histidyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 TIGR00442: histidine--tRNA ligase" amino acids 18 to 458 (441 residues), 388.7 bits, see alignment E=1.6e-120 PF13393: tRNA-synt_His" amino acids 21 to 366 (346 residues), 152.4 bits, see alignment E=1.8e-48 PF03129: HGTP_anticodon" amino acids 385 to 489 (105 residues), 45.3 bits, see alignment E=8.1e-16

Best Hits

Swiss-Prot: 100% identical to SYH_CAUVC: Histidine--tRNA ligase (hisS) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 100% identity to ccs:CCNA_03628)

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3CFG4 at UniProt or InterPro

Protein Sequence (495 amino acids)

>CCNA_03628 histidyl-tRNA synthetase (Caulobacter crescentus NA1000)
MTTDSEQPNTDFRPEARAPRGFADKRARDLRAERAILEAVSAVYERYGFEALDTGAFEYA
DALGKFLPDSDRPNEGVFALQDDDDQWMALRYDLTAPLARFAAQNWETLPKPFRRYAFGP
VWRNEKPGPGRFRQFIQCDADTVGSARPEADAEIIAMAVEGLEAAGLPRGTAVLKINNRK
LLNGLLTAAGADSAGQKLAVLRAVDKLDRLGVDGVRLLLGEGRLDESGDFTKGAGLKGKA
VDQVLDFVQAGSTGGRAATLDNIARVVAGSAEGDEGLLELSRIDAALSSLGIADDQAFID
PSIVRGLEYYTGAVFEAELLLSTTDEKGNKVSFGSIGGGGRYDDLVARFTGNVTPATGFS
FGVSRLAAALRAAGREPGGAARGPVVVITFDQAHMGEYFSVVGQLRAAGIAAEVYLGTSG
MKPQMKYADRRGAPAVVMLGGDEIAAGTVTIKDLDAGRMAAEGLTDNAAWKAERPGQQTI
PRDQLVDAVRKIIGG