Protein Info for CCNA_03317 in Caulobacter crescentus NA1000

Annotation: DNA polymerase III alpha subunit DnaE2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1083 PF02811: PHP" amino acids 10 to 126 (117 residues), 40.9 bits, see alignment E=7.1e-14 TIGR00594: DNA polymerase III, alpha subunit" amino acids 172 to 1010 (839 residues), 446.7 bits, see alignment E=9.6e-138 PF07733: DNA_pol3_alpha" amino acids 276 to 531 (256 residues), 295.8 bits, see alignment E=8.2e-92 PF17657: DNA_pol3_finger" amino acids 534 to 709 (176 residues), 173.7 bits, see alignment E=5.4e-55 PF14579: HHH_6" amino acids 783 to 891 (109 residues), 44.3 bits, see alignment E=4.1e-15 PF01336: tRNA_anti-codon" amino acids 975 to 1050 (76 residues), 41.9 bits, see alignment 1.9e-14

Best Hits

Swiss-Prot: 100% identical to DNAE2_CAUVC: Error-prone DNA polymerase (dnaE2) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

Predicted SEED Role

"Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)" (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8H427 at UniProt or InterPro

Protein Sequence (1083 amino acids)

>CCNA_03317 DNA polymerase III alpha subunit DnaE2 (Caulobacter crescentus NA1000)
MRPPVYAELQATTNFSFLRGASHAEELALTAEALGLTAIGIVDRNSLAGVVRAWTAAKRR
SIRVLTGCRLDFMDGAPSLLCYPTDREAFGRLTRLLTIGQLRAEKGECHLTWRDFLDHSE
GQLGLIVPPRVLDDSFEQHLTRMAGDLRGRSWLAASRAYAARDLQRLARLESLGRTSGAP
IVATNDVLYHGPERRPLQDVISCVREHCAIQEAGFRLEANAERHIKSPEEMARLFDRWPR
AVERTVEIVERIGFDLKDIREQYPDEPVPPGKTAMQHLTDLTWKGAAWRYPNGVSPKVTA
QIQEELRLIEKMDYPNYFITVHDIVREARSMGILCQGRGSAANSSVCFCLGVTAIDPTEH
RLLFTRFISENRGEPPDIDVDFEHDRREEVMQYVFQRYGRAYAAICGTVIHYRPRSAIRD
VGKALGLTEDVTSLLAGTVWGSWGDGLPEDHLRNAGLDPKAPEIARAVGLANDLIGFPRH
LSQHVGGFVLTKRRLDETVPIGKAAMKDRTFIEWDKDDIDSLGLMKVDILALGMLHAIQR
AMTMLREDHGQDWLKDLADIPKEVPGVYDMLCAADSVGVFQVESRAQMSMLPRLRPREFY
DLVIQVAIVRPGPIQGDMVHPYLKRRNKEEPVDWPKPSPEHGPPDELQEILGKTFGVPLF
QEQAMSLAIEAAKFTPDEADGLRKAMATFRNLGSPDAYRNKFIEGMVGRGYERAFAERCF
KQIEGFSHYGFPESHAASFAKLVYVSAWIKWAWPDVFCAALINAQPMGFYQPAQLVRDAR
EHGVEVLPPDILTSDWDCTLAPISTGFRPPRVRHDKVACQETRPRWKAVRLGFRQIKGLR
ETLDIPPLLKARGEGARTPAEFAQGGVPQKALELLAEADAFASVGLSRREALWAVKGLKG
EHKAPVQAPLLAGLPLFEERVALPAMAATQEVAEDYRTTSLSLKAHPIGFYRSMLAARGV
VPAERLLSLKDGARVSVAGLVLIRQRPGTAKGVVFVTLEDETGVANAVVWKDRFDAARNV
VMTASFLIVHGRVQRADNVIHVVAERFTDLSAELSSLRDEPGAPAPRIRQKVSGRLLRSR
DFH