Protein Info for CCNA_03124 in Caulobacter crescentus NA1000

Annotation: NAD/mycothiol-dependent formaldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08240: ADH_N
  • amino acids 24 to 153 (130 residues), 94.1 bits, E=9e-31
• PF00107: ADH_zinc_N
  • amino acids 195 to 320 (126 residues), 100 bits, E=1.5e-32
• PF13602: ADH_zinc_N_2
  • amino acids 229 to 356 (128 residues), 32.6 bits, E=2.1e-11

Best Hits

KEGG orthology group: K00121, S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase [EC: 1.1.1.1 1.1.1.284] (inferred from 100% identity to ccr:CC_3029)

Predicted SEED Role

"Alcohol dehydrogenase (EC 1.1.1.1)" in subsystem Fermentations: Mixed acid or Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 1.1.1.1)

MetaCyc Pathways

• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• ethanol degradation II (3/3 steps found)
• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• acetaldehyde biosynthesis I (1/1 steps found)
• phytol degradation (3/4 steps found)
• L-isoleucine degradation II (2/3 steps found)
• L-leucine degradation III (2/3 steps found)
• L-valine degradation II (2/3 steps found)
• ethanol degradation I (1/2 steps found)
• pyruvate fermentation to ethanol II (1/2 steps found)
• protein S-nitrosylation and denitrosylation (3/5 steps found)
• L-phenylalanine degradation III (2/4 steps found)
• L-tyrosine degradation III (2/4 steps found)
• salidroside biosynthesis (2/4 steps found)
• heterolactic fermentation (12/18 steps found)
• serotonin degradation (4/7 steps found)
• L-methionine degradation III (1/3 steps found)
• pyruvate fermentation to ethanol I (1/3 steps found)
• pyruvate fermentation to ethanol III (1/3 steps found)
• noradrenaline and adrenaline degradation (8/13 steps found)
• phenylethanol biosynthesis (2/5 steps found)
• cytidine-5'-diphosphate-glycerol biosynthesis (1/4 steps found)
• mixed acid fermentation (9/16 steps found)
• (S)-propane-1,2-diol degradation (1/5 steps found)
• acetylene degradation (anaerobic) (1/5 steps found)
• ethanolamine utilization (1/5 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (11/19 steps found)
• butanol and isobutanol biosynthesis (engineered) (3/8 steps found)
• superpathway of N-acetylneuraminate degradation (12/22 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (2/9 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (4/13 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (4/17 steps found)
• L-tryptophan degradation V (side chain pathway) (1/13 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Drug metabolism - cytochrome P450
• Fatty acid metabolism
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Metabolism of xenobiotics by cytochrome P450
• Methane metabolism
• Retinol metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.1, 1.1.1.284

Use Curated BLAST to search for 1.1.1.1 or 1.1.1.284

Sequence Analysis Tools

See A0A0H3CBH0 at UniProt or InterPro

Protein Sequence (366 amino acids)

>CCNA_03124 NAD/mycothiol-dependent formaldehyde dehydrogenase (Caulobacter crescentus NA1000)
MKAAVLREVGKPLQIETVAIGKPGPREVLIRTKAAGVCHSDLHFVEGSYTHALPAVLGHE
SAGIVEAVGSEVRTVKVGDHVITCLNPYCGHCEVCLTGHMNLCISPETRRSKSDAPRLFK
EDLNGATGPMAQFLNLSSFAEMMLVHEHACVAIRKDMPFDRAALIGCSVMTGVGAVMHTS
NVRPGETVAVIGCGGVGLATINGAAIAGAGRIIAIDRLAGKLELAKTFGATDVVDASQVD
DIAKAVVELTGGGVHHSFEAIGLKATAEASFKMLRRGGTANVIGMIPVGTKIELHGVDFL
GERRIQGSYMGSNRFPVDMPRLVDFYMSGKLKLDELISRRIKLEDVNSAFDELKRGELAR
SVIVFD