Protein Info for CCNA_03115 in Caulobacter crescentus NA1000

Annotation: cobaltochelatase cobS subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 336 TIGR01650: cobaltochelatase, CobS subunit" amino acids 8 to 334 (327 residues), 730.4 bits, see alignment E=1.2e-224 PF12556: CobS_N" amino acids 15 to 47 (33 residues), 56.6 bits, see alignment 2.5e-19 PF07728: AAA_5" amino acids 74 to 196 (123 residues), 43.5 bits, see alignment E=4.9e-15

Best Hits

Swiss-Prot: 72% identical to COBS_SINSX: Aerobic cobaltochelatase subunit CobS (cobS) from Sinorhizobium sp.

KEGG orthology group: K09882, cobaltochelatase CobS [EC: 6.6.1.2] (inferred from 100% identity to ccr:CC_3021)

MetaCyc: 72% identical to CobS (Pseudomonas denitrificans (nom. rej.))

Predicted SEED Role

"Aerobic cobaltochelatase CobS subunit (EC 6.6.1.2)" in subsystem Coenzyme B12 biosynthesis or Conenzyme B12 related Hypothetical: Clusters with cobST (EC 6.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.6.1.2

Use Curated BLAST to search for 6.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3CCB1 at UniProt or InterPro

Protein Sequence (336 amino acids)

>CCNA_03115 cobaltochelatase cobS subunit (Caulobacter crescentus NA1000)
MPEFAETSSAADPLITLVPDRWVTLRDAFGVDSDMKVPAFSHRDSHVPDIDPAYRFDPQT
TKAICAGFAYDRRVMVQGYHGTGKSTHIEQIAARLNWPLVRVNLDSHVSRIDLVGKDAIV
LKDGKQITEFREGILPWTLQRPVALVFDEYDAGRPDVMFVIQRVLEAGGKLTLLDQNRVI
RANPYFRLFATTNTIGLGDTTGLYHGTQQINQGQMDRWSIVTTLNYLEHDVEAAIVLAKN
PGFDSVEGKRTISAMVRVADMTRNAFMNGDISTVMSPRTVITWAQNAEIFDHDVALAFRL
TFLNKCDELERPTVAEFFQRAFGTDLPESAARVKVA